潮州地区高危型HR-HPV感染与子宫颈病变的关系

Evaluate the relation of subtypes of high risk human papillomavirus and cervical disease in Chaozhou area

目的探讨潮州地区高危型人乳头瘤病毒(HR-HPV)感染与子宫颈病变的关系。方法采用实时荧光定量PCR技术对潮州农村35～60周岁的妇女进行HR-HPV检测,对HR-HPV阳性妇女进行核酸分子快速导流杂交分型技术(HybriMax)和液基薄层细胞学(LCT)检查,对低级别鳞状上皮内病变(low grade squamous intraepithelial lesions,LSIL)及以上患者行阴道镜下组织病理学活检确诊。结果潮州地区妇女HR-HPV感染率为8.25%(4 080/49 458)。发现15种HR-HPV,感染率最高的型别是HPV52,其次为HPV16、58、68、33和18型。HPV16、HPV59和混合型感染者子宫颈病变较严重,与其他型别感染者子宫颈病变程度差异有显著性(P<0.05)。结论潮州地区人群感染HR-HPV优势型别为HPV52,HR-HPV不同型别与子宫颈病变严重程度密切相关。

Purpose To evaluate the relation of subtypes of high risk human papillomavirus（HR-HPV） and cervical disease in Chaozhou area.Methods HR-HPV test by real-time fluorescent quantitative PCR（FQ-PCR） was used to the women aged from 35 to 60 who joined in the new rural cooperative medical care in Chaozhou area.the HybriMax and LCT was only used to HR-HPV positive cases.The histopathologic biopsy was used to the LSIL and worse cases.Results The infection rate of HR-HPV among women in Chaozhou area was 8.25%（4 080/49 458）.15 high risk subtypes were detected.The subtype with the highest infection rate was HPV 52（30.55%）,followed by HPV 16,58,68,33 and 18.HPV16,HPV59 and Mixed types infection had worse cervical disease which were higher than those with other subtype of HPV.Conclusions The predominant subtype of HPV infection was HPV52 in Chaozhou area,the different subtype of HPV were significantly associated with the degree of cervical disease.