基因芯片筛查多囊卵巢综合征来源胚胎干细胞分化的脂肪细胞中的差异基因

Differential Gene Screening on Fat Cells Differentiated from PCOS Serived Human Embryonic Stem Cells (hESCs) by Microarray

目的:筛选出与多囊卵巢综合症(PCOS)相关的特异表达基因,以期阐明PCOS的发病机制。方法:将本实验室自主建立的PCOS来源和非PCOS来源的人胚胎干细胞(hESCs)系定向分化成脂肪细胞,应用北京博奥生物有限公司的晶芯?人类全基因组寡核苷酸微阵列芯片检测不同来源脂肪细胞中的差异基因。结果:PCOS来源和非PCOS来源的人胚胎干细胞均成功诱导分化成了脂肪细胞,共筛选出2种脂肪细胞中的差异表达基因153个,其中PCOS来源脂肪细胞上调表达91个,下调表达62个。挑选上调表达的6个基因进行Real-time PCR验证,与基因芯片检测结果一致。结论:PCOS和非PCOS来源hESCs分化的脂肪细胞在基因表达上有差异,这些差异基因很多涉及糖、脂代谢,并与甾体激素相关,进一步研究后有部分基因可作为PCOS发生、发展的候选基因。

Objective： To explore the molecular mechanisms of pathogenesis of PCOS and provide clues and basis for clinical diagnosis and treatment. Methods： Three human embryonic stem cells （hESCs） lines derived from PCOS （p-hESC-1, p-hESC-2, p-hESC-3） and one hESCs line derived from non-PCOS （ZZU-hESC-1）were directionally differentiated into fat cells. Beijing Boao Crystal Core human whole-genome oligonucleotide microarraywas applied to screen for differential genes between fat cells differentiated from PCOS and non-PCOS hESCslines. Results： All of hESCs had been differentiated into fat cells. A total of 153 differentially expressed genesbetween PCOS and non-PCOS derived fat cells were found according to the result of microarray analysis,including 91 up-regulated expression genes and 62 down-regulated expression genes in PCOS derived fat cells.Real-time PCR was used to validate the six differential genes. The results were accordant with microarray results.Conclusion：Those differential genes are widely distributed, involving glucose, lipid and steroid metabolism, avariety of diseases and so on. Some of them can be served as candidate genes for PCOS occurrence anddevelopmentin the further study.