中药生精散对精子DNA完整性的影响及其在IVF-ET治疗中的临床疗效

Influence of Shengjingsan(Traditional Chinese Medicine) Prescription on Spermatozoa DNA Integrity and Clinical Effects on IVF-ET Treatment

目的:观察中药生精散对不育患者精子DNA完整性的影响及其在IVF-ET治疗中的临床疗效。方法:因少、弱精子症行IVF/ICSI-ET治疗的患者49例,随机分为中药组(22例,服用生精散治疗)和对照组(27例,未经生精散治疗)。观察比较中药组和对照组精液常规分析(精子密度、活率、活力)、DNA碎片指数(DNA fragmentation index,DFI)、受精率、卵裂率、种植率及临床妊娠率。结果:中药治疗后精子活率及活力显著提高(P<0.05),且中药组DFI值较治疗前及对照组明显下降(P<0.05)。中药组胚胎种植率(31.11%)及临床妊娠率(45.45%)明显高于对照组(13.73%和18.51%)(P<0.05)。结论:中药生精散可通过降低不育患者精子DNA损伤程度,改善精子质量,提高IVF-ET的治疗效果。

Objective：To observe the influence of Traditional Chinese Medicine（TCM）—— Shengjingsan on infertile spermatozoa DNA integrity and clinical effects in IVF-ET treatment. Methods： A total 49 cases were recruited, patients in TCM group underwent 22 cases treated by Shengjingsan for 2-3 courses, patients incontrol group underwent 27 cases without any medicine. Outcome parameters were conventional semen parameters（sperm density, motility rate and vitality）, DNA fragmentation index（DFI）, the number of retrieved oocytes, thenumber of mature eggs, the rates of fertilization, cleavage, implantation and clinical pregnancy. Results： For TCMgroup, sperm motility rate and vitality were significantly higher after treating by Shengjingsan as compared with thembefore （P0.05）, and DFI was significantly lower in TCM group than that in control （P0.05）. Implantation rate（31.11% vs 13.73%, P0.05） and clinical pregnancy rate （45.45% vs18.51%, P0.05） were significantly higher inTCM group than those in the control, respectively. Conclusion： The Traditional Chinese Medicine—— Shengjingsanprescription can reduce sperm DFI of the infertile patients and improve the sperm quality, and also increase the clinical pregnancy rate and embryo implantation rate in IVF-ET cycles.