狂犬病病毒抗体免疫金标检测试纸的制备及其应用

Preparation and application of colloidal gold strip for the detection of antibodies against rabies virus

为了建立一种快速检测狂犬病病毒抗体水平的方法,用浓缩提纯的狂犬病病毒CVS11株作为胶体金标记抗原,以金黄色葡萄球菌A蛋白为检测线上的捕获抗原,制备狂犬病病毒抗体水平检测试纸条。应用该试纸条进行临床样品检测,结果表明,以稀释后的犬全血或血清作为检测样品,试纸条检测的灵敏度为0.002U/mL,特异性试验证明该试纸条与犬细小病毒、犬瘟热病毒、犬腺病毒2型犬科疾病阳性血清没有交叉反应,稳定性试验证明该试纸条在室温下保存的有效期为12个月,检测结果与快速荧光抑制试验的符合率为95.12%。证实该狂犬病病毒抗体免疫金标检测试纸可以方便、快速、灵敏、特异地检测狂犬病病毒的抗体水平。

In order to develop a new method for rapid detection of antibodies against rabies virus,strips were prepared with the principle of colloidal gold immunochromatography assay.The purified rabies virus CVS11 strain was labeled by colloidal gold and SPA was used as the test capture reagent to detect the corresponding antibodies.Clinical samples were tested by the colloidal gold strip with the lowest test level of 0.002 U/mL.The strip did not cross react with the positive sera of canine parvovirus,canine distemper virus and canine adenovirus type 2.The strip was still effectual after stored for 12 months at room temperature.The coincidence rate between the colloidal gold strip and RFFIT was 95.12%.The result showed that the colloidal gold strip for rabies virus antibody detection was sensitive,specific,convenient to be used and rapid to get results.