摘要
金纳米棒具有独特的光学性质与良好的生物适应性,在生物医学领域得到了日益广泛的应用。利用种子生长法,通过改变表面活性剂十二烷基二甲基苄基氯化铵(BDAC)的浓度所制备的8种不同长径比(AR)的金纳米棒,具有不同位置的纵向表面等离子共振(SPR)峰。在金纳米棒和非标记探针DNA的混合盐溶液中加入靶标DNA后检测,发现金纳米棒的纵向SPR峰会发生一定的"蓝移",并伴有明显的吸光度下降;且研究发现该体系检测下限为纳摩尔水平。
Gold Nanorods have been widely used in biomedical field, due to their unique optical properties and excellent biocompatibilities. Eight different aspect ratios （AR）gold nanorods which own different longitudinal surface plasmon resonance modes is described. Gold nanorods are prepared with the seed-mediated growth method with various concentration of benzyldimethylhexadecylammonium chloride（BDAC）. While target DNA is added to the mixed buffer of nonmodified gold nanorods and label-free probe DNA with high ionic strength, the ＂blue- shifted＂ of gold nanorods ＇ SPR modes can be detected immediately, and obvious absorbance deductions are observed. This method shows high sensitivity which allows detection of target DNA at a concentration as low as nanomolar range.
出处
《传感器与微系统》
CSCD
北大核心
2011年第10期137-140,共4页
Transducer and Microsystem Technologies
基金
卫生部科技重大专项"十一五"计划资助项目(2008ZX10004-002)
教育部中央高校基本科研业务费专项资金资助项目(10D10515)
上海市科委科技攻关计划资助项目(09391911500)
关键词
金纳米棒
分子检测
表面等离子共振
吸光度
gold nanorod（GNR）
molecular detection
surface plasmon resonance（SPR）
absorbance
