升阳十一味丸对胃癌细胞凋亡及细胞周期的影响

Effect of Shengyangshiyiweiwan on Cell Apoptosis and Cell Cycle in Gastric Cancer

研究复方蒙药升阳十一味丸对体外培养的人胃癌MGC—803细胞凋亡及细胞周期的影响。以不同浓度的蒙药处理MGC—803细胞,通过MTT法检测药物作用后细胞的增殖情况,并计算生长抑制率。利用倒置光学显微镜、Hochest33342/PI荧光染色、流式细胞术,研究蒙药诱导细胞凋亡的作用及对细胞周期的影响。结果蒙药能抑制MGC—803细胞的增殖,并具有良好的量效关系。2.5 mg/mL药物作用MGC—803细胞48 h后,光镜下可见核固缩、核碎裂等凋亡细胞形态学变化。流式细胞仪检测到"亚G1峰",细胞周期分析表明S期细胞较对照组增多,G2/M期细胞下降,阻滞于S期。说明蒙药升阳可明显抑制胃癌MGC—803细胞的增殖,并在一定浓度范围内诱导细胞凋亡,这可能是其发挥抗肿瘤的作用主要途径之一。

To studay the effect of Inner Mongolia Drug Shengyangshiweiwan on cell apoptosis and cell cycle in humand gastric cancer MGC—803 cell,MTT assay was used to examine the proliferation inhibitory effect of shengyangshiyiweiwan on MGC—803 cell,and calculate the inhibitory rate of growth.Morphologic changes of apoptosis were observed by reverse discrepancy and offluorescent light microscope;flow cytometry was used to analysis cell cycle.MTT assay showed that shengyang has proliferation inhibiting effect on MGC—803 cell in dose and time dependent manners.The cells treated with shengyang（2.5 mg/mL） for 48 hours showed characters of apoptosis under light microscope.An ＇sub-G1 peak was detected by flow cytometry,the result analysis of cell cycle indicated that the number of cells of S phase increased,that of G0/ G1 and G2/M phase decreased after treatment on MGC—803,it was arrested S phase.It is conclused that shengyang has proliferation inhibiting effect and may induce apoptosis in gastric cancer MGC—803 cell in a certain dose range,which may be one of the antitumor mechansim.