红景天苷对荷瘤小鼠抗肿瘤活性和免疫功能的研究

Study of the Antitumor Activity and Immunity Enhancement of Salidroside in Vivo

研究红景天苷抗肿瘤活性及其提高免疫功能的机制。用胃腺癌BGC-823在BALB/c小鼠建立皮下肿瘤模型。分别用大、中、小剂量红景天苷灌胃,以环磷酰胺(CTX)为阳性对照组,连续给药12 d后处死小鼠,称小鼠体质量、瘤重,计算脾脏系数,同时测定脾淋巴细胞转化率和IL—2活性。各剂量红景天苷给药组均能显著提高小鼠的体质量,且能明显抑制荷瘤小鼠肿瘤生长,以大、中剂量组比较明显,而环磷酰胺组体质量明显下降(P<0.05)。各红景天苷给药组均能提高脾脏系数和脾脏细胞功能,增加脾淋巴细胞转化率和IL—2活性,以大剂量给药组作用最明显,而环磷酰胺阳性对照组的体液免疫功能则显著下降。红景天苷能明显抑制BGC—823肿瘤的生长,同时能增加小鼠体质量,提高治疗肿瘤的药物耐受性,改善体液免疫功能而发挥抗癌作用,高效低毒,可与化疗药物合用提高其疗效。

To investigate the antitumor activity and immunity enhancement of salidroside, the tumor model was established using subcutaneous BGC--823 cell injection in BALB/c mice, and the animals were divided into the salidroside treatment groups （high, middle and low concentration）, and cyclophosphamide （CTX） treatment group was positive control as while saline treatment group was negative control. The mice bodies and tumors were weigh- ted and the spleen coefficient was measured. Spleen lymphocyte conversion ratio and IL--2 activity were also meas- ured. Comparing to negative control group, the mice body weight were significantly higher and the tumor weight were significantly lower in salidroside treatment groups, but the body weight markedly decreased in the group trea- ted with CTX （P 〈 0. 05 ）. And their thymus and spleen coefficient were higher than negative control groups. Sali- droside increased spleen lymphocyte conversion ratio and IL--2 value, but all the above value were significantly decreased in CTX treatment group. Salidroside can obviously inhibit BGC--823 tumor growth by increasing the body weight of mice, enhancing the immunity, which can improve the drug tolerance of patients.