Hypothesis for metabolites of denitrifying phosphate accumulating organisms by the restriction of denitrifying bacteria in anoxic phase

The objective of this work is to verify a hypothesis that nitrite accumulation comes from the metabolites of denitrification phosphate accumulating organisms (DPAOs),not denitrifying bacteria.On the precondition of the restriction of denitrifying bacteria in anoxic phase,static experimental test was designed using NO3-as electron acceptor,effluent was removed after sedimentation in anaerobic phase,and the same concentration solution of PO43--P was returned,so that TOC was excluded and denitrification was inhibited in the next phases.A parallel experiment was carried out simultaneously with the normal anaerobic-anoxic progress.The results showed that,in static test,by keeping the normal growth of DPAO and inhibiting denitrification of denitrifying bacteria,P-release in anaerobic and P-uptake in anoxic phase proceeded normally.DPAO had obvious effect on P-removal and the P-removal efficiency was 69%.The effluent concentration of NO3--N and NO2--N was 7.62 mg/L and 6.05 mg/L respectively,compared with parallel experiments,and nitrogen removal rate was lower.No nitrite residue was found in parallel test.Therefore,it can confirm the hypothesis that the metabolites of DPAO are both nitrogen and nitrite when nitrate is taken as electron acceptor,and nitrite is subsequently converted to nitrogen by denitrifying bacteria.

The objective of this work is to verify a hypothesis that nitrite accumulation comes from the metabolites of denitrifieation phosphate accumulating organisms （DPAOs） , not denitrifying bacteria. On the precondition of the restriction of denitrifying bacteria in anoxic phase, static experimental test was designed using NO3 - as electron aeceptor, effluent was removed after sedimentation in anaerobic phase, and the same concentration solution of PO43- -P was returned, so that TOC was excluded and denitrification was inhibited in the next pha- ses. A parallel experiment was carried out simultaneously with the normal anaerobic-anoxic progress. The re- suits showed that, in static test, by keeping the normal growth of DPAO and inhibiting denitrification of denitrif- ying bacteria, P-release in anaerobic and P-uptake !n anoxic phase proceeded normally. DPAO had obvious effect on P-removal and the P-removal efficiency was 69%. The effluent concentration of NO3 - -N and NO2 - -N was 7.62 mg/L and 6.05 mg/L respectively, compared with parallel experiments, and nitrogen removal rate was lower. No nitrite residue was found in parallel test. Therefore, it can confirm the hypothesis that the metabolites of DPAO are both nitrogen and nitrite when nitrate is taken as electron acceptor, and nitrite is subsequently convened to nitrogen by denitrifying bacteria .