碘化N-正丁基氟哌啶醇对大鼠心肌细胞肌浆网钙泵的保护作用

The Protectivie Effects of N-n-butyl Haloperidol Iodide on Sarcoplasmic Reticulum Ca^(2+)-ATPase of Cardiac Myocytes in Rats

目的:研究碘化-N-正丁基氟哌啶醇(F2)对大鼠心肌细胞肌浆网钙泵(SERCA)的保护作用。方法:通过结扎成年SD大鼠冠状动脉左前降支造成急性心肌缺血再灌注(I/R)模型,舌下静脉给予不同剂量(0.3、0.5、1.0、2.0和4.0mg/kg)F2,测定SERCA的活性和蛋白表达变化。结果:I/R组SERCA活性为对照组的57.7%、不同剂量F2组分别为67.1%、72.8%、77.4%、84.1%和86.7%。结论:F2能量效-依赖地减轻I/R导致的SERCA活性降低。

Objective： To investigate the protective effects of N-n-butyl haloperidol iodide on sarcoplasmic reticulum Ca^2＋-ATPase（SERCA）of cardiac myoeytes in rats. Methods： The experimental models of rots were established by occluding left anterior descending coronary artery, and intravenous various doses of 172 before I/R. The activity of SERCA was observed by tested kits and the quantity of SERCA was tested. Results： The activity of SERCA decreased after IfR. SERCA was reduced to 57.7 % （ P 〈 0.05）. Intravenous 172 at 0.3 、0.5, 1. 0、2.0 and 4.0 mg/kg before isehemia reduced SERCA to67.1%、72.8%, 77.4%、84.1%、86.7% respectively. The quantity of SERCA had no changes. Conclusion： Intravenous F2 in rats could ameliorate the decreased activity of SERCA dose-deoendently.