纳米微载体介导的低氧诱导因子-1圈套寡核苷酸对小鼠HepG_2细胞血管内皮生长因子的作用

The Effect of Hypoxia-inducible Factor-1 Decoy Oligodeoxynucleotide Mediated by Nano Micro Carrier on Vascular Endothelial Growth Factor in HepG2 Cells of Mice

目的:以低氧诱导因子(HIF)-1为靶点,观察HIF-1转录因子圈套寡核苷酸(HIF-1 decoy ODN))对小鼠HepG2细胞内血管内皮生长因子(VEGF)的作用,在分子水平为肝细胞癌干预靶点提供实验依据。方法:用ELISA法测定小鼠HepG2细胞内VEGF浓度。用逆转录-聚合酶链式反应测定小鼠HepG2细胞内VEGFmRNA。结果:HIF-1 decoy ODN在体外能有效抑制HIF-1活性。DecoyODN治疗后小鼠HepG2细胞内VEGF表达明显下降,而变异的decoyODN无效果。结论:纳米微载体介导的HIF-1 decoy ODN能下调HepG2细胞的VEGF水平。

Objective： To investigate the effect of hypoxia-inducible factor-l（HIF-1）decoy oligodeoxynucleotide（ODN） mediated by nano micro cartier on vascular endothelial growth factor（VEGF） in HepG2 cells of mice. Methods： ELISA was used to detect the contents of VEGF and RT-PCR to detect the mRNA of VEGF. Results： The HIF-1 decoy ODN could effectively inhibit the activity of VEGF in vitro. After treatment with decoy ODN, the expression of VEGF significandy decreased. Conclusion： The HW -1 decoy ODN mediated by nano micro carrier cart decrease the VEGF in HepG2 cells of mice.