大剂量米非司酮对子宫内膜腺组织中缺氧诱导因子-1α仪及血管上皮生长因子表达的影响

The effect of high-dose mifepristone on the expression of HIF-la and VEGF in the endometrial tissue

目的通过大剂量米非司酮对子宫内膜腺组织中缺氧诱导因子-1α（HIF-lα）及血管上皮生长因子（VEGF）表达的影响，探讨米非司酮治疗子宫内膜癌的分子机制。方法选择确诊子宫内膜癌患者30例，手术前口服米非司酮100mg／d，顿服，分别留取服药有活检或诊刮及术中组织标本作为研究对象，另选择正常子宫内膜10例作为对照组。采用免疫组化法检测HIF-1α理及VEGF的表达。结果子宫内膜癌组织HIF-1α（78．6±3．5）及VEGF（92．4±5．1）表达明显高于正常子宫内膜组织HIF-1α[（192．3±4．7），P〈0．01]及VEGF[（178．4±3．3），P〈0．01]，大剂量米非司酮可明显降低子宫内膜癌组织HIF-1α（103．2±4．1）及VEGF的表达（118．3±4．9）（P〈0．05）。结论降低HIF-1α及VEGF的表达可能是米非司酮抑制子宫内膜癌的一个分子机制。

Objective To study the effect of high-dose mifepristone on the expression of hypoxia-induciblefactor 1, （HIF-lα） and vascular endothelial growth factor（VEGF） in the endometrial tissue and research the molecular mechanisms of mifepristone in treat ment of endometfial cancer. Methods 30 patients with endometrial cancer who were treated were asked to oral mifepristone 100mg/d,the specimens from a biopsy or curettage were taken as the group before treatment,the specimens from surgery were as the group of after treatment. Another 10 cases of normal endometrium were selected as the control group. The expression of HIF-lα and YEGF were examined with immunohistochemistry. Results The expression of HIF-1 ct and VEGF （78.6± 3.5,92.4 ~ 5.1 ） in endometrial cancer was sig- nificantly higher than normal endometrium （ 192.3 ± 4.7,178.4 ±3.3, P 〈 0.01 ） ,large doses of mifepristone can significantly reduce the expression of HIF-1± and VEGF in endometrial cancer issues （103.2± 4.1,118.3 ± 4.9, P 〈 0. 05）. Conclusion One of the molecular mechanisms of mifepristone inhibits the endometrial cancer was reduced HIF-1± and VEGF expression.