一株Ⅰ型鸭肝炎病毒全基因组序列分析

Whole-genome analysis of one strain of Duck hepatitis virus type Ⅰ

为了研究Ⅰ型鸭肝炎病毒(DHV-Ⅰ)遗传变异情况,试验采用全基因组序列测定的方法对广东省佛山地区分离到的1株Ⅰ型鸭肝炎病毒进行分析研究。结果表明:不含poly(A)尾的DHV-Ⅰ基因组全长7 690 bp,只含有1个开放阅读框(ORF),编码2 249个氨基酸;将此病毒株与GenBank公布的其他DHV全基因序列进行序列分析,VP1蛋白变异最大,180～220位为高变区,与DHV-Ⅰ参考毒株核苷酸序列同源性在94.0%～97.4%之间,氨基酸序列同源性在97.5%～99.2%之间;对此病毒株进行遗传进化分析,与DHV-Ⅰ参考毒株处于同一分支,与韩国和中国台湾省新型鸭肝炎病毒处于不同分支。

To investigate the variation and genetic evolution of Duck hepatitis virus type Ⅰ,the genome sequence of Duck hepatitis virus which isolated from foshan was analyzed.The results show that the full length of genome was 7 690 bp,including one single open reading frame（ORF） encoding a polypeptide of 2 249 amino acids（aa）.Compared to the other genome of DHV-Ⅰ from GenBank,VP1 was the largest variation.The hypervariable regions were from 180 to 220 aa.The amino acid sequences shared 97.5% to 99.2% amino acid identity with those of classical DHV-Ⅰ.The whole nucleotide sequence shared 94.0%-97.4% identity with those genomes.The isolate was situated in the DHV-Ⅰ branch of the phylogenetic tree and was less related with Taiwan strains and Korean strains which were in different branches.