Ki-67与p53在肺硬化性血管瘤中的表达及其临床意义

Expressions of Ki-67 and p53 in pulmonary sclerosing hemangioma and the clinical significance

目的分析Ki-67与p53蛋白在肺硬化性血管瘤（PSH）表面被覆细胞和间质多边形细胞中的表达以及Ki-67、p53蛋白与PSH的生物学行为的关系。方法随机数字表法抽取21例PSH患者手术切除标本，应用免疫组织化学双标法检测21例标本Ki-67和CK8／18蛋白表达，免疫双荧光组织化学染色技术检测9例p53和AE1／AE蛋白的表达。结果CK8／18和AE1／AE3定位于细胞膜，表达于表面被覆细胞；Ki-67与p53定位于细胞核，在两种细胞均见表达。其中Ki．67在表面被覆细胞中表达阳性率〈1％，在间质多边形细胞中阳性率为1％～10％；p53主要表达于间质多边形细胞（单信号6例），仅个别被覆细胞阳性（双信号3例）。结论PSH中的间质多边形细胞和表面被覆细胞具有形态和免疫表型的差异，间质多边形细胞的增殖指数和基因突变均高于被覆细胞，PSH的生物学行为可能主要决定于间质多边形细胞。

Objective To study the expressions of Ki-67 and p53 in the surface cells and polygonal cells in pulmonary sclerosing hemangioma （PSH） and investigate the relation of cell proliferation index and biological behaviour of the tumor. Methods Double-staining immunohistochemistry was used to detect the expressions of Ki-67 and CK8/18 protein. Double immunofluorescence staining was used to detect the expressions of p53 and AE1/AE3 protein. Results The positive signal of AE1/AE3 and CK8/18 were localized in cytomembrane of surface cells. The positive signal of Ki-67 and p53 were localized in cell nucleus of the two kinds of cells. The positive rate of Ki-67 was under 1% in surface cells and 1%-10 % in polygonal cells, p53 protein was mainly expressed in polygonal cells （6/9, 33.3 %） and only exsited in seldom surface cells. Conclusion There are differences on cellular morphous and immunophenotype between the surface cells and polygonal ceils. The proliferation index and gene mutation are all predominant in polygonal ceils than in surface cells. The biological behaviour of PSH maybe mainly be decided by the polygonal cells.