供者外周血干细胞动员采集198例临床分析

Clinical analysis on the mobilization and collection of peripheral hematopoietic stem cells in 198 healthy donors

目的研究外周血造血干细胞移植（PBSCT）供者外周血干细胞动员采集的方法及其效果。方法198名健康供者每天皮下注射重组人粒细胞集落刺激因子（rhG—CSF）（5—10）ug／kg进行外周血干细胞动员，第5天开始采集。采用血细胞分析仪行单个核细胞（MNC）计数，流式细胞术（FCM）行CD34＋细胞计数。分析供者性别、身高、年龄、采集当天外周血白细胞（WBC）计数对动员采集效果的影响。结果所有供者均成功动员采集，采集当天的MNC计数平均为（4．19±1．96）×10^8／kg，CD34＋细胞计数平均为（2．98±1．40）×10^6／kg；MNC和CD34＋细胞计数与供者性别、身高、年龄无关；采集当天外周血WBC计数与MNC、CD五细胞计数呈正相关（r=0．9201，P=0．0035；r=0．8420，P=0．0149）；采集当天外周血WBC计数≥20．0×10^9／L的供者比〈20．0×10^9／L的供者采集效果更显著（F=4．688，P=0．0013；F=4．622，P=0．0006）。结论rhG-CSF动员的健康供者采集当天外周血WBC计数是一项预测CD34＋胞采集数量的简单、可行的指标。

Objective To investigate the methods and effects on the mobilization and collection of peripheral hematopoietic stem cells. Methods Peripheral hematopoietic stem cells of 198 cases of healthy donors were selected and mobilized by subcutaneous recombinant human granulocyte colony stimulating factor （rhG-CSF） by （5-10） ug·kg-1·d-1 and collected on the 5th day. The effects of gender, height, age and WBC count of donors on mobilization and collection were analyzed. Results All the peripheral hematopoietic stem cells of donors were successfully mobilized. The average counts of mononuclear cells （MNC） and CDL cells were （4.19±1.96）×10^8/kg and （2.98±1.40）×10^6/kg, respectively, which had no correlation with gender, height and age. The counts of MNC and CD ;4 cells collected were positively correlated with the WBC count of peripheral blood （r =0.9201, P =0.0035; r =0.8420, P =0.0149）. The donors with WBC ≥20.0×10^9/L had more significant effect than those WBC〈20.0×10^9/L （F =4.688, P =0.0013; F =4.622, P =0.0006）. Conclusion The WBC count of peripheral blood from healthy donors is a simple and feasible indicator to predict the quantity of CD34＋ cells.