抗日本血吸虫pVAX1/SjHGPRT·SDISP多价疫苗对昆明小鼠的保护作用及其机制探讨

Immunoprotection and Mechanism Exploration of Multivalent DNA Vaccine pVAX1/SjHGPRToSDISP Against Schistosoma Japonicum

目的:探讨抗日本血吸虫生殖产卵编码基因多价疫苗pVAX1/SjHGPRToSDISP对昆明小鼠的保护作用及其机制。方法:选取昆明小鼠30只,分别使用pVAX1/SjHGPRToSDISP、pVAX1以及生理盐水,每只小鼠100μg或等量经左腿股四头肌注射。处理2周后,采集动物模型血样检测IgG、IL-2,IL-4,IL-10以及INF-γ表达量。处理4周后,以20±1条尾蚴贴腹感染,感染6周后检测减虫率、减卵率。结果:尾蚴攻击动物后6周,pVAX1/SjHGPRToSDISP免疫组的肝脏减虫率为42.2%,子宫与肝脏减卵率分别为68.04%以及72.96%。与对照组比较,差异有显著性。pVAX1/SjHGPRToSDISP免疫组虫体内IgG、IL-4以及INF-γ表达量明显升高,与对照组比较差异有显著性(P<0.05)。结论:pVAX1/SjHGPRToSDISP多价疫苗具有较好的免疫保护作用,且该类作用的机制与IgG、IL-4以及INF-γ的表达升高存在关联。

Objective： To explore the protection of multivalent DNA vaccine pVAX1/SjHGPRToSDISP in Kunming mice and it＇s mechanism. Methods： A total of 30 Kunming mice were selected and treated by pVAX1/SjHGPRToSDISP, pVAX1 and normal saline respectively. Each mouse was injected with 100 ug or equivalent amount through quadriceps of left legs. Two weeks later after the injection, the serum of the mice was selected to detect the expression level of IgG, IL-2, IL-4, IL-10, INF-y . After 4 weeks of the injection, the mice were infected by the procercoid through abdominal wall. After the infection for 6 weeks, the worm reduction rate and the egg reduction rate were investigated. Results： The worm reduction rate of the pVAX1/SjHGPRT. SDISP was 42.2%. The egg reduction rates of the pVAX1/SjHGPRToSDISP in uterus and liver were 68.04% and 72.96%,respectively, which were significantly different from the control group （P〈0.05）. The expression level of IgG, IL-4 and INF-y in pVAX1/SjHGPRToSDISP immune group is significantly higher than that of the control group （P〈0.05）. Conelusion： The pVAX1/SjHGPRToSDISP showed good immnunoprotection, which was correlated with the increased expression level of IgG, IL-4, INF-y .