通络药物对心梗后心衰大鼠非梗死区心肌纤维化的影响

Effect of Qiliqiangxin Capsule on Myocardial Fibrosis in Non-infarct Zone of Rats with Heart Failure After Myocardial Infarction

目的:探讨不同剂量芪苈强心胶囊对心衰模型大鼠非梗死区胶原蛋白分子表达的影响。方法:将通过结扎冠状动脉左前降支并饲养4周的56只心衰模型鼠随机分成4组:心衰对照组(MI-C)、转换酶抑制剂雷米普利治疗组(MI-R,10 mg/kg.d)、芪苈强心小剂量组(MI-S,0.25 g/kg.d)以及芪苈强心大剂量组(MI-L,1.0 g/kg.d)。同步药物干预4周后,ELISA法检测Ang II水平、RT-PCR检测非梗死区胶原-I mRNA。结果:血清中Ang II的浓度:与心力衰竭对照组比较,假手术组、雷米普利组、大剂量芪苈强心组和小剂量芪苈强心组均明显降低(P<0.05)。其中,大剂量芪苈强心组比雷米普利组明显减低,差异具显著性(P<0.05);而小剂量芪苈强心组与雷米普利组水平接近,差异无显著性(P<0.05)。非梗死区胶原-I mRNA的表达:与心衰对照组比较,假手术组、雷米普利组、大剂量芪苈强心组,小剂量芪苈强心组表达均下调,差别具显著性(P<0.05);大剂量芪苈强心组与雷米普利组接近,差别无显著性(P>0.05);小剂量芪苈强心组高于大剂量芪苈强心和雷米普利组,差别具有显著性(P<0.05)。结论:芪苈强心胶囊能够明显地减少心梗后心衰非梗死区胶原分子的合成,并具有明显的剂量依赖性。

Objective： To investigate the effect of different dosages qiliqiangxin capsule on the production of collagen in non-infarct zone （NIZ）. Method： A total of 56 rats surviving left anterior descending coronary artery were randomly divided into placebo （Ml-control）, Ramipril （MI-ramipril, 10 mg/kg.d） therapy, qiliqiangxin large dosage （MI-large, 1.0 g/kg.d） therapy and qiliqiangxin small dosage （MI-small, 0.25 g/kg.d） therapy with additional eight other rats being sham. Four weeks later, the plasma angiotensin II （Ang II） was detected by using ELISA, and the expressions of collagen types I （collagen I） were measured by reverse transcription-polymerase chain reaction （RT-PCR）. Results： Plasma Ang 1I concentration evidently dropped in the MI-ramipril rats, MI- large and MI-small rats, com- pared with that in the control rats. Compared with that in MI- ramipril rats, plasma Ang II concentration decreased significantly in MI- large rats （P 〈0.01 ）. There was no difference between ramipril rats and MI-small rats. The expressions of collagen I and total collagen concentration in NIZ were as follow： Compared with that in sham rats, the expressions of collagen I and total collagen concentration in MI-control rats and the other three admission groups increased evidently （P〈0.05）. Compared with that in MI-control rats, the proteins were down-regu- lated in MI-ramipril rats and MI-large rats. There were no significant differences between MI-ramipril rats and MI-large rats （P〉0.05）. And there were no differences between MI-control rats and MI-small rats（P〉0.05）. Conclusions： Qiliqiangxin Capsule can significantly inhibit the production of collage in NIZ, which is in a dose-dependent manner.