南昌地区多重耐药肺炎链球菌的分子分型

Molecular typing of multidrug resistant Streptococcus pneumoniae in Nanchang district

目的了解南昌地区多重耐药肺炎链球菌(Streptococcus pneumonia,SP)的分子流行病学特征。方法收集南昌地区3家教学医院临床分离的SP 66株,用盒式聚合酶链反应(BOX-PCR)对23株临床分离的SP进行基因分析;用多位点序列分型(multilocus sequence typing,MLST)对其中7株多重耐药SP进行分子遗传学分析,了解其与国际流行株关系。结果 23株SP用BOX-PCR检测共分为11型,15个亚型。7株多重耐药SP MLST分型共检出7种序列型,分别为ST320、ST236、ST983、ST790、ST5747、ST5748、ST5749,其中ST5747、ST5748、ST5749为国际上首次发现。结论南昌地区多重耐药SP增加是由于耐药克隆株的播散,主要克隆株来自国际流行克隆CC236(Taiwan19F-14 Clone)的传播。

Objective To investigate multiple drug-resistance of Streptococcus pneumonia（SP） of clinical isolates and the molecular epidemiology in Nanchang district.Methods Sixty-six specimens of SP isolates were collected from three teaching hospitals in Nanchang district.Among them 23 clinical isolates of SP were genotyped by BOX-PCR and their genetic relationship were analyzed.The evolutional relationship of 7 multidrug resistant strains was investigated by multilocus sequence typing（MLST） and the comparison with international epidemic strains was carried out.Results The 23 SP strains were examined and divided into 11 distinct types,and 15 subtypes by BOX-PCR.The 7 multidrug resistant strains were identified as 7 sequence type（ST） by MLST,i.e.,ST320,ST236,ST983,ST790,ST5747,ST5748 and ST5749,among which ST5747、ST5748、ST5749 were first reported in the world.Conclusion The high prevalence of multidrug resistant strains should be caused by the dissemination of drug-resistant strain clone and the main clone is the international clone CC236（Taiwan19F-14 clone）.