H.pylori-L型感染及OPN表达与胃癌浸润转移的相关性研究

Relationships between Helicobacter pylori L-forms infection and expression of OPN and invasion and metastasis of gastric carcinoma

目的通过检测胃癌中幽门螺杆菌L型(Helicobacter pylori L-form,H.pylori-L型)感染以及骨桥蛋白(osteopontin,OPN)的表达情况,探讨H.pylori-L型在胃癌侵袭、转移中的作用。方法将胃癌BGC-823细胞与H.pylori-L型按不同比例共培养,细胞爬片进行免疫组化(SP法)检测BGC-823细胞OPN的表达情况。革兰染色及免疫组化检测120例胃癌组织中H.pylori-L型的感染情况。免疫组化检测胃癌组织中的OPN的表达。结果 (1)随着细菌比例增大,细胞爬片OPN阳性的胃癌BGC-823细胞数逐渐增加,各组之间差异有统计学意义(F=137.5,P<0.01)。(2)胃癌组织H.pylori-L型阳性组的OPN表达阳性率为70.73%,显著高于H.pylori-L型阴性组OPN表达阳性率42.1%(P<0.005)。胃癌中H.pylori-L型阳性率与肿瘤的大小、癌细胞的血管侵袭、浸润深度及淋巴结转移具有相关性(P<0.001～P<0.05)。(3)胃癌组织中H.pylori-L型阳性率与OPN的阳性表达呈正相关性(r=0.27,P<0.001)。结论 H.pylori-L型感染增强了胃癌细胞的侵袭和转移能力,其机制可能与胃癌细胞的OPN表达量增加有关。

Objective To detect Helicobacter pylori L-form（H.pylori-L） infection and the expression of OPN in gastric carcinoma,and discuss the role of H.pylori-L infection in the progression of gastric carcinoma.Method Gastric cancer BGC-823 cells were co-incubated with H.pylori-L in different proportions;The expressions of OPN in BGC-823 cells were assessed by immunohistochemical staining.H.pylori-L infection and OPN expression were detected by Gram＇s staining and immunohistochemical staining in 120 cases of gastric carcinoma tissues.Result The cells of OPN positive arised with the increasing of H.pylori-L concentrations（F=137.5,P0.01）.In gastric carcinoma tissues,H.pylori-L infection had positive correlation with OPN expression（r=0.27,P0.001）.The infection of H.pylori-L was related to the size,invasion and metastasis of the tumors（P0.001～P0.05）.Conclusiont Infection of H.pylori-L may increase the invasion and metastasis ability of stomach carcinoma cells,and the increase of expression levels of OPN may be one of the mechanisms.