生物膜定量分析仪对不同细菌早期生物膜形成能力的比较研究

Comparison of early formation of biofilm in different bacterial strains by biofilm quantitative analysis

目的通过生物膜定量分析仪来观察铜绿假单胞菌(Pseudomonas aeruginosa PAO1),变形链球菌(Streptococcus mutans UA159)以及大肠埃希菌(Escherichia coli MG1655)生物膜形成能力的不同,并以各菌株的吸光度值A600为参考,对3种菌株早期生物膜形成能力进行比较。方法通过向生物膜培养悬液中加入与细菌直径相近的磁性小珠,利用这些小珠在磁场中受到生物膜的位移约束力的原理,采用生物膜定量分析仪,定量比较3种菌株在生物膜形成上的差别。结果实验发现铜绿假单胞菌PAO1和大肠埃希菌MG1655的细菌增长速度基本相同,但铜绿假单胞菌PAO1的生物膜形成明显快于大肠埃希菌MG1655。大肠埃希菌MG1655和变形链球菌UA159的生物膜形成速度基本相同,但大肠埃希菌MG1655的细菌增长速度明显高于变形链球菌UA159。结论不同细菌有各自的生物膜形成模式。生物膜定量分析仪作为一种高效简便的检测手段,可用于生物膜早期形成的动态分析。

Objective To compare the biofilm early formation ability of Pseudomonas aeruginosa PAO1,Streptococcus mutans UA159 and Escherichia coli MG1655 through a biofilm quantitative analysis named BioFilm Ring Test.Method Based on th eimmobilization of magnetic beads by adherent cells,an assay was developed for the kinetic quantification of biofilm formation in this study.Result There were no significant difference between Pseudomonas aeruginosa PAO1 and Escherichia coli MG1655 in bacterial growth rate.But the biofilm formation speed of Pseudomonas aeruginosa PAO1 was much quicker than that of Escherichia coli MG1655.There were no significant difference between Escherichia coli MG1655 and Streptococcus mutans UA159 in biofilm formation speed.But the bacterial growth rate of Escherichia coli MG1655 was much higher than that of Streptococcus mutans UA159.Conclusion Each bacterial strain has its own special pattern of biofilm formation.The BioFilm Ring Test may be used as a rapid,reproducible and easy-handling method to study the kinetics of bacterial biofilm early formation.