摘要
目的:探讨同型半胱氨酸(Hcy)及其代谢关键酶蛋氨酸合成酶还原酶(methionine synthase reductase,MTRR)基因多态性与颅内动脉瘤的关系。方法:采用化学发光技术检测76例颅内动脉瘤患者(颅内动脉瘤组)和77例健康成人(对照组)的血浆Hcy水平,并利用聚合酶链反应-限制性内切酶片段长度多态性技术(PCR-RFLP)检测其代谢关键酶MTRR基因多态性。结果:颅内动脉瘤患者血浆Hcy水平[(16.14±6.72)μmol/L]明显高于对照组[(11.20±3.20)μmol/L],两组间血浆甘油三酯(TG)和总胆固醇(TC)水平差异无显著性。两组MTRRA66G基因型分布比较,颅内动脉瘤组GG基因型及G等位基因频率均明显高于对照组,患颅内动脉瘤的风险是对照组的7.30和2.41倍。MTRR A66G不同基因型颅内动脉瘤患者比较,血浆TG、TC浓度差异无显著性,GG基因型血浆Hcy浓度较其他基因型高;对照组不同基因型间血浆Hcy、TG、TC水平差异均无显著性。结论:高Hcy血症与颅内动脉瘤病发生有一定关系;MTRR A66G的AA基因型可能是颅内动脉瘤的保护性基因,发生变异时患病风险增加。
Objective To explore relationship of plasma homocysteine（Hcy） level and polymorphism of methionine synthase reductase （MTRR） with intracranial aneurysm. Method The genotyes of MTRR were determined by PCR-based assay and their plasma Hcy levels were determined in 76 patients with intracranial aneurysm and 77 healthy controls. Results Mean total plasma Hcy concentrations were significantly higher in intracranial aneurysm patients than in the normal subjects. Aneurysm group MTRR A66G AA genotype and A allele frequencies were higher than the normal （P 0.01）, the risk of intracranial aneurysm patients is 7.30 and 2.41 times than the control group. MTRR A66G genotype TC, TG, Hcy concentration group comparison showed that different genotypes aneurysm group TG, TC showed no significant difference （P 0.05）, AA genotype with high Hcy levels than other genotypes, with significant significance （P 0.05）, aneurysm group Hcy concentrations than the control group, with significant differences （P 0.05）, but the two groups of TG and TC levels were not significantly different （P 0.05）, the control group between the different genotypes TG, TC, Hcy levels were not significantly different （P 0.05）. Conclusions Hyperhomocysteinemia may be independent risk factors for intracranial aneurysm and MTRR A66G of the AA genotype may be the independent genetic risk factors of intracranial aneurysms.
出处
《实用医学杂志》
CAS
北大核心
2011年第19期3522-3525,共4页
The Journal of Practical Medicine
