肝舒胶囊对小鼠的免疫调节作用

Effect of Ganshu capsule on immune function of mice

目的探讨肝舒胶囊的免疫调节作用。方法测定环磷酰胺(CY)免疫抑制小鼠的血清溶血素和正常小鼠的血清溶血素;测定氢化可的松(HC)免疫抑制小鼠网状内皮系统吞噬功能碳粒的廓清指数;测定二硝基氯苯(DNCB)诱导小鼠迟发型超敏反应小鼠耳的肿胀度。结果 1.9、0.95 g.kg-1肝舒胶囊对CY所致小鼠血清溶血素低下有显著提高;1.9 g.kg-1肝舒胶囊对正常小鼠血清溶血素有提高的作用趋势、能明显提高HC免疫抑制小鼠血清碳粒廓清指数、对DNCB致小鼠迟发型超敏反应有明显抑制。结论肝舒胶囊对免疫抑制小鼠体液免疫及非特异性免疫功能均有明显的增强作用;对细胞免疫有一定的抑制作用。

OBJECTIVE To study the immuno-modelating effects of Ganshu capsule in mice.METHODS Serum hemolysin test was used to observe the level of serum hemolysin in normal mice and immune hypofunction mice induced by cyclophosphamide,carbon particle clearance method was used to investigate the monocyte cytophagic index in normal mice and immune hypofunction mice induced by hydrocortisone,delayed type hypersensitivity（DTH） test was used to observe the level of ear swlling in normal mice and immune hypofunction induced by dinitrochlorobenzene（DNCB）.RESULTS Ganshu capsule（1.9,0.95 g·kg-1） could elevate the serum hemolysin level in immunosuppressed mice induced by cyclophosphamide,and Ganshu capsule（1.9 g·kg-1） elevated the serum hemolysin level in normal mice but the difference was insignificant.Ganshu capsule（1.9 g·kg-1） could increas the monocyte cytophagic index in immunosuppressed mice induced by hydrocortisone,and markedly inhibit dimethylbenzene-induced ear swelling.CONCLUSION Ganshu capsule significantly improves nonspecific and specific immunologic function,and inhibits cellular immunologic function in immuno-suppressed mice.