期刊文献+

HPLC同时测定兔血浆中的丙泊酚及其前药HX0969w 被引量:3

Simultaneous determination of Propofol and its prodrug HX0969w in rabbit plasma by HPLC
原文传递
导出
摘要 目的采用HPLC法同时测定兔血浆中的丙泊酚及其前药HX0969w。方法采用Agilent Eclipse-Plus C18色谱柱(150mm×4.6 mm,5μm);流动相为甲醇-水-10%四丁基氢氧化铵(70∶29∶1,加入0.1%三乙胺,磷酸调pH7),流速1 mL.min-1;HX0969w的检测波长为260 nm,丙泊酚为272 nm。结果 HX0969w与丙泊酚的线性范围分别为46.5~2973.6μg.mL-1(r=0.9997)、9.4~601.0μg.mL-1(r=0.9993);平均回收率分别为95.97%、98.08%,日内RSD≤4.22%,日间RSD≤7.92%(n=5)。结论新建方法可简便、准确、迅速地同时测定血浆中前药HX0969w和丙泊酚的含量,可用于HX0969w药物动力学的初步研究和原料与制剂中前药和丙泊酚的含量测定。 OBJECTIVE To establish an HPLC method for simultaneously determining the concentration of Propofol and its prodrug HX0969w in rabbit plasma.METHODS HPLC method was adopted.An Agilent eclipse plus C18 column(150 mm×4.6 mm,5 μm) was used.The mobile phase consisted of methanol-water-10% tetrabutyl ammonium hydroxide(70:29:1,with 0.1% triethylamine,adding phosphoric acid to adjust pH7.0).The flow rate was 1 mL·min-1,the detection wavelength was 260 nm for HX0969w and 272 nm for propofol.RESULTS The linear range of HX0969w was 46.5-2973.6 μg·mL-1(r=0.9997),the average recovery was 95.97 %.The linear range of propofol was 9.4-601.0 μg·mL-1(r=0.9993),the average recovery was 98.08%.The intra and inter-assay precision for this analysis were less than 4.22% and 7.92%,respectively.CONCLUSION The method is simple,accurate,rapid,and can be used for the simultaneous determination of propofol and its prodrug HX0969w in plasma.Thus the method is suitable for pharmacokinetic studies of HX0969w and the content detection of the raw material or preparation.
出处 《华西药学杂志》 CAS CSCD 北大核心 2011年第5期490-492,共3页 West China Journal of Pharmaceutical Sciences
关键词 前药 HX0969w 丙泊酚 高效液相色谱法 Prodrug HX0969w Propofol HPLC
  • 相关文献

参考文献5

  • 1Scott RP, Saunders DA, Norman J. Propofol: clinical strategies for preventing the pain of injection [ J ]. Anaesthesia, 1988,43 ( 6 ) : 492 - 494.
  • 2Tan CH,Onsiong MK. Pain on injection of Propofol [J]. Anaesthesia, 1998,53 ( 5 ) :468 - 476.
  • 3Baker MT, Naguib M. Propofol: the challenges of formulation [J]. Anesthesiology, 2005,103 ( 4 ) : 860 - 876.
  • 4Wolf A, Weir P, Segar P, et al. hnpaired fatty acid oxidation in Propofol infusion syndrome [ J ]. Lancet,2001,357 ( 9256 ) :606 - 607.
  • 5Parke TJ,Stevens JE, Rice AS,et al. Metabolic acidosis and fatal myocardial failure after Propofol infusion in children :five case reports [ J ]. British Med J, 1992,305 ( 6854 ) :613 - 616.

同被引文献11

引证文献3

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部