东北玉簪多糖提取分离及组成研究

Study on the Isolation and Composition of Hosta ensata Polysaccharides

[目的]研究玉簪多糖的提取分离及其组成。[方法]利用水提醇沉法得粗多糖,并依次用30%、50%、80%乙醇分部沉淀得多糖CP1、CP2、CP3;经Superdex-100凝胶柱色谱分离得CP4、CP5。采用光谱法和色谱法对其总糖含量、单糖组成等进行了分析。[结果]东北玉簪5个多糖样品酸性糖含量为20.36%~52.76%、中性糖含量为34.07%~55.62%。[结论]东北玉簪多糖CP1~CP5均为中性糖和酸性糖的混合物。

[Objective]To study the isolation and composition of polysaccharides from Hosta ensata.[Method]Crude polysaccharide was obtained by water extraction and then precipitated respectively by 30%,50% and 80% alcohol to get CP1,CP2 and CP3,CP4 and CP5 were isolated through Superdex G-100 gel column chromatography.Content of total sugar and composition of monose were analyzed by using spectrum and chromatography.[Result]Of five polysaccharide samples from Hosta ensata,the content of acidic polysaccharides ranged from 20.36% to 52.76%,while that of neutral polysaccharides was in the range of 34.07%-55.62%.[Conclusion]All CP1-CP5 from Hosta ensata were the mixture of acid and neutral sugar.