醋酸去氧皮质酮高血压大鼠动脉结构与功能的变化

Changes of arterial structure and function in hypertensive rats induced by deoxycorticosterone acetate salt

目的:探讨盐敏感性高血压大鼠大动脉结构和功能的变化。方法:12周龄SD大鼠(23只)被分为假手术组(10只)和醋酸脱氧皮质酮(DOCA)盐高血压组(13只),观察其外周及中心动脉压,脉搏波速度(PWV)、颈总动脉内膜中层厚度(IMT)和胸主动脉中层厚度(MT)的改变,并分析血管壁胶原/弹性蛋白组分的变化。结果:与假手术组相比,造模结束后DOCA高血压大鼠外周收缩压明显升高[(110.69±4.19)mmHg比(166.58±4.28)mmHg,P<0.05],中心收缩血压(cSBP),中心舒张血压(cDBP),中心平均血压(cMBP)亦显著升高(P均<0.01),同时伴有胸主动脉PWV明显升高[(4.81±0.14)m/s比(6.33±0.20)m/s,P<0.01]。DOCA盐高血压大鼠颈动脉IMT[(46.00±4.00)μm比(78.00±4.90)μm]和主动脉中层厚度(MT)[(120.48±7.93)μm比(194.25±5.66)μm]明显增厚(P均<0.01),血管壁胶原含量亦明显升高[(0.87±0.05)mg/cm比(1.00±0.03)mg/cm,P<0.05]。结论:醋酸脱氧皮质酮盐高血压大鼠存在明显的血压升高和动脉僵硬;除血压影响之外,其动脉僵硬度的改变亦与大动脉管壁增厚及胶原含量变化有关。

Objective： To explore changes of large arterial structure and function in salt sensitive hypertensive rats. Methods： A total of 23 12-week--old SD rats were randomly divided into sham operation group （n： 10） and hypertension induced by deoxycortieosterone acetate （DOCA） salt group （n= 13） . Changes of their peripheral and central arterial pressure, pulse wave velocity （PWV）, intima-media thickness （IMT） of carotid and thoracic aorta was observed. Changes of components of collagen/elastin on vessel wall were analyzed. Results： Compared with sham operation group, peripheral systolic blood pressure [SBP, （110.69±4. 19） mmHg vs. （166.58±4. Z8） mmHg, P〈0.05] significantly increased, and there were also significant increase in central SBP （cSBP）, central diastolic blood pressure （eDBP）, central mean blood pressure （eMBP）, P〈0.01 all, and PWV of thoracic aorta [ （4.81±0. 14） m/s vs. （6.33±0.20） m/s, P〈0.01] also significantly increased in DOCA rats at the end of modeling. Carotid IMT [ （46.00 ±4.00） μm vs. （78.00±4.90） μm] and aortic media thickness （MT） [ （120.48±7.93） vm vs. （194.25±5.66） μm] significantly thickened （P〈0.01 both） , and collagen concentration on vessel wall [ （0.87±0.05） mg/cm vs. （1.00±0.03） mg/cm, P〈0.05] also significantly increased in DOCA rats. Conclusion： The blood pressure and artery stiffness in DOCA- salt- hypertensive rats significantly increasetly change of arterial stiffness is related with thicken of large arterial wall and change of collagen concentration besides impact from blood pressure.