温阳通督中药结合理筋手法对颈脊髓机械性压迫损伤家兔模型诱发电位的影响

Influence of Chinese medicine combined with tendon-soothing manipulation on evoked potential in rabbit model of cervical spinal cord injury induced by mechanical compression

目的探讨温阳通督中药结合理筋手法对颈脊髓机械性压迫损伤家兔模型诱发电位的影响。方法选用新西兰大白兔60只,在家兔C5椎体上植入不锈钢螺钉,建立家兔颈脊髓机械性压迫损伤模型,术后观察2月并进行Tarlov评分后,拍摄X线片以验证造模情况。造模成功后,将上述家兔随机分为中药加手法组、中药组、甲钴胺组和对照组,对中药加手法组、中药组、甲钴胺组分别进行手法及药物干预。干预2月后使用RM6240型多道电生理仪检测家兔脊柱脊髓诱发电位和外周神经脊髓诱发电位。结果中药加手法、中药和甲钴胺3种干预手段对家兔外周神经脊髓诱发电位和脊柱脊髓诱发电位影响不同,中药加手法组和甲钴胺组外周神经脊髓诱发电位的增幅大于对照组(P<0.05),中药加手法组脊柱脊髓诱发电位的增幅大于对照组(P<0.05)。结论温阳通督中药结合理筋手法,能够改善因机械性压迫所导致的脊髓诱发电位改变,具有修复脊髓损伤、增强神经传导速度的作用。

Objective To investigate the influence of Chinese medicinal with actions of warming yang and dredging Du meridian combined with tendon-soothing manipulation on the evoked potential in rabbit model of cervical spinal cord injury induced by mechanical compression. Methods The model of cervical spinal cord injury induced by mechanical compression was established in 60 rabbits by implantation of stainless bolts into C5. After observing for 2 months and Tarlov scoring, the modeling result was examined with X-ray films. The rabbits were randomly divided into the Chinese medicinal and manipulation group, Chinese medicinal group, mecobalamin group and control group after successfully modeling. The Chinese medicinal and manipulation group, Chinese medicinal group and mecobalamin group were intervened by the manipulation and Chinese medicinal. The evoked potential of spinal cord and evoked potential of peripheral nerve spinal cord were detected by using RM6240 electrophysiologic instrument after intervention for 2 months. Results The influences of Chinese medicinal and manipulation, Chinese medicinal and mecobalamin were different on the evoked potential of peripheral nerve spinal cord and evoked potential of spinal cord. The amplification of the evoked potential of peripheral nerve spinal cord was higher in the Chinese medicinal and manipulation group and mecobal- amin group than that in the control group （P 〈 0. 05 ）. The amplification of the evoked potential of spinal cord was higher in the Chinese medicinal and manipulation group than that in the control group （P 〈 0. 05）. Conclusion Chinese medicinal with actions of warming yang and dredging Du meridian combined with tendon-soothing manipulation have the effect of repairing spinal cord injury, promoting nerve conduction velocity, and relieving the evoked potential changes of spinal cord induced by mechanical compression.