通气对光合自养培养下蛋白核小球藻生长和有机碳成分含量的影响

The effects of aeration on the growth and organic carbon component contents under photoautotrophy condition

为了研究通气速率、气体的CO2浓度、通气阶段对小球藻光合自养生长和小球藻有机碳成分含量的影响,分别进行了未通气体、通入空气、通入纯CO2(99.9%)条件下的小球藻光合自养培养试验。试验发现,通入空气速率为1 L/min时小球藻的生长速率最快;高浓度CO2对小球藻生长有抑制作用;在接种初期进行通气试验更有利于小球藻的生长。不同通气条件下小球藻的有机成分含量无明显差异,在通空气和通纯CO2条件下的小球藻有机碳组分中,除总脂肪酸含量有所降低外,其余有机碳组分含量都有所升高。由于通入空气有利于小球藻的生长,所以通气条件有利于提高小球藻的固碳。

The experiment conditions of without aeration, air aeration （0.03% CO2 concentration）, pure CO2 aeration （99.9% CO2 concentration）in photoautotrophy cultivation were designed respectively for the analysis that the effect of air aeration rate, CO2 concentration, aeration phases on photoautotrophy growth and organic carbon component content of C. pyrenoidosa. It showed that the air aeration rate of 1 L/min was optimal for cell growth; high CO2 concentration would inhibit the cell growth; air aeration was beneficial to the growth of cell at the beginning of inoculation. It was also found that there were no obvious variation of organic component content under different aeration conditions, organic carbon component content except the total fatty acids content had little increased under the conditions of air aeration and pure CO2 aeration. Above all, air aeration condition was benefit for alga growth and CO2 fixation efficiency.