高产生物丁醇新菌株的筛选、鉴定及发酵研究

Screening、identification and fermentation of a new strain for producing butanol

丁醇是具有巨大应用潜力的可再生能源。为了获得高效生产丁醇的菌株,通过玉米培养基富集、丁醇耐受筛选的方法,从自然环境(牛粪、沼气池和土壤)来源的60份样品中分离获得1株丁醇发酵比率高的新菌株,菌株命名为gxzp-13-2。经梯度浓度葡萄糖发酵试验表明,5%葡萄糖发酵丁醇产量较高,最高可达10.05 g/L,总溶剂达到13.95 g/L,丁醇比约为72.1%,转化率为31.8%。对此菌株进行了分子鉴定,经16S rDNA基因序列同源性分析表明,gxzp-13-2与拜氏梭菌(Clostridium beijerinckii)的同源性最高,相似性高达98%。gxzp-13-2在厌氧固体培养基上生长,菌落呈紫色。该菌株为下一步的菌种基因改造工作提供了研究基础,也为丁醇发酵提供了宝贵的菌种资源。

Butanol is a renewable energy source of great potential. In order to obtain high efficient strains of producing butanol, one purple bacterial colony was screened by the method of enriching corn medium and tolerant selection from 60 samples, which were got from cow dung, methane tank and soil. The new strain could effectively produce butanol,which named as gxzp-13-2. The results of fermentation by gradient concentration of glucose showed： the yield of butanol fermented by 5% of glucose was highest, which reached 10.05 g/L, the total solvent was 13.95 g/L, the rate of butanol was 72.1% and conversion was 31.8%. It had the highest homology （98%） with Clostridium beijerinckii through 16S rDNA sequence analysis.Gxzp-13-2 grew on anaerobic medium,the colony was purple. The strain could not only provide basis for alternating genes of strains for further study, but also provide valuable stains resource for fermentation butanol.