正交试验法优选安神颗粒的醇提工艺

Optimization of Alcohol Extraction Technology for Anshen Granules by Orthogonal Test

目的:优选安神颗粒的醇提工艺。方法:以酸枣仁皂苷A、五味子醇甲为指标,以HPLC测定,用Lichrospher C18色谱柱(4.6 mm×250 mm,5μm),柱温30℃,流速1.0 mL.min-1;酸枣仁皂苷A流动相乙腈-水(30.5∶69.5),检测波长204 nm;五味子醇甲流动相甲醇-水溶液(13∶7),检测波长250 nm。采用正交试验法,优选醇提工艺。结果:乙醇用量与提取次数对方中五味子醇甲的提取有显著性影响。此复方中五味子、酸枣仁药物最佳醇提工艺为每次用10倍药材量的85%乙醇提取2次,每次1.0 h。结论:该提取工艺合理,生产实际操作简单,适合于大生产。

Objective：To optimize alcohol extraction process of Anshen granules.Method： The optimum alcohol extraction conditions were investigated by orthogonal design with the content of jujuboside A and schizandrin as indexes.Determined by HPLC,using Lichrospher C18column（4.6 mm×250 mm,5 μm）,column temperature was 30 ℃,flow rate was 1.0 mL·min-1;Determined jujuboside A with CH3CN-water（30.5∶69.5）as mobile phase,the UV detection wavelength was 204 nm;Schizandrin were investigated with methanol-water（13∶7）as mobile phase,the UV detection wavelength was 250 nm.Result： The amount of ethanol and extracting time had significant effect on extraction of schizandrin.The optimum alcohol extraction technology of Schisandrae Chinensis Fructus and Ziziphi Spinosae Semen from complex prescription was as follows：extracted 2 times with 10 times amount of 85% ethanol for 1.0 h per time.Conclusion：This extraction technology is reasonable,simple and suitable for mass production.