HPLC测定活络效灵颗粒中芍药苷和丹酚酸B的含量

Content Determination of Paeoniflorin and Salvianolic Acid B in Huoluoxiaoling Granules by HPLC

目的:建立测定活络效灵颗粒中2种有效成分芍药苷和丹酚酸B含量的方法。方法:采用高效液相色谱法,芍药苷以Zorbax SB-C18(4.6 mm×150 mm,5μm)色谱柱,以乙腈-水(10∶90)为流动相同,检测波长230 nm,柱温为40℃,流速为1.0 mL.min-1。丹酚酸B以Zorbax Extend-C18(4.6 mm×150 mm,5μm)色谱柱,以甲醇-乙腈-0.5%甲酸(22∶10∶68)为流动相,检测波长286 nm,柱温为25℃,流速1 mL.min-1。结果:芍药苷和丹酚酸B分别在0.084 4～0.928 4μg(r=0.999 8)和0.226～2.034μg(r=0.999 9)线性关系良好;平均回收率分别为100.4%(RSD 2.30%)和100.7%(RSD 2.73%)。结论:该方法简便易行、重复性好,可用于活络效灵颗粒中芍药苷和丹酚酸B含量测定。

Objective：To establish the method for the content determination of paeoniflorin and salvianolic acid B in Huoluoxiaoling granules.Method：HPLC was adopted to determine the content of paeoniflorin and salvianolic acid B.The column for paeoniflorin is Zorbax SB-C18（4.6 mm×150 mm,5 μm）.The mobile phase is consisted of acetonitrile-water（10∶90）with the detection wavelength at 230 nm.The column temperature was at 40 ℃ and the flow rate was set at 1.0 mL·min-1.The column for salvianolic acid B is Zorbax Extend-C18（4.6 mm×150 mm,5 μm）.The mobile phase is consisted of methanol-acetonitrile-0.1% formic acid（22∶10∶68）with the detection wavelength at 286 nm.The column temperature was at 25 ℃ and the flow rate was set at 1.0 mL·min-1.Result：There was good linearity between the peak area and concentration within the range of 0.084 4-0.928 4 μg（r=0.999 8）for paeoniflorin and 0.226-2.034 μg（r=0.999 9）for salvianolic acid B respectively.The average recoveries of paeoniflorin and salvianolic acid B were 100.4%（RSD 2.30%）and 100.7%（RSD 2.73%）respectively（n=6）.Conclusion：The method is simple,accurate and specific for quality control of Huoluoxiaoling granules.