山东不同栽培品种金银花HPLC指纹图谱的比较

Study on HPLC Fingerprint of Lonicerae Japonicae Flos from Shandong

目的:建立并比较山东不同栽培品种金银花的HPLC指纹图谱,为考察其质量并对其品种鉴定与划分建立图谱标准。方法:采用HPLC,以乙腈和1%醋酸水溶液进行梯度洗脱。使用"中药色谱指纹图谱相似度评价系统"和SPSS软件进行数据处理,对不同栽培品种金银花的HPLC指纹图谱进行了相似度和聚类分析。结果:建立了山东不同栽培品种金银花的HPLC对照指纹图谱。结论:山东产不同栽培品种金银花的指纹图谱有一定差异,可为金银花的质量考察、品种鉴定及划分提供一定的参考依据。

Objective：To study the HPLC fingerprint of Lonicerae Japonicae Flos and establish a chromatographic standard for the quality control as well as to comparative study the HPLC fingerprint among various farm cultivars.Method： The HPLC method was used.The gradient elution was adopted acetonitrile and 1% acetic acid.The ＇similarity evaluation system for chromatographic fingerprint of TCM＇ algorithm was adopted in combination with the clustering analysis to study ten flos lonicerae＇s thirteen characteristic common peaks,and to evaluate the quality and dividide the farm cultivars of flos lonicerae.Result： The HPLC fingerprint of Lonicerae Japonicae Flos were established and the differences of HPLC fingerprint among the farm cultivars of Lonicerae Japonicae Flos produced in Shandong were compared.Conclusion： The HPLC fingerprint method summaried in this article can be used as the typical chromatography and provide conference evidence for dividing the Cultivars resource of Lonicerae Japonicae Flos.