活血化瘀方清除DPPH自由基活性量效关系

Dose-response Relationship of Free Radical Scavenging Activity by Blood-activating and Stasis-removing Formulae

目的:对活血化瘀代表性方剂进行清除2,2-二苯基-1-苦肼基(DPPH)自由基活性评价。方法:采用DPPH自由基清除率法测定活血化瘀代表方血府逐瘀汤(XF)、少腹逐瘀汤(SF)、膈下逐瘀汤(GX)、身痛逐瘀汤(ST)、通窍活血汤(TQ),每个组方分别取全方、醇沉沉淀和醇沉上清液3个不同样品测清除DPPH自由基活性。结果:活血化瘀方各样品对DPPH自由基都有明显的清除作用,与空白比较有显著性差异(P<0.01),且有明显的量效关系,按生药量比较,5个全方较各分离部位效果好,全方清除自由基活性顺序为:GX>TQ>SF>ST>XF。其IC50按生药量计依次为0.93,1.40,1.50,2.31,2.32 g.L-1。结论:采用酶联免疫检测仪检测活血化瘀方对DPPH自由基的清除作用,方法简便,重复性好,适合高通量筛选中药及方剂的清除自由基活性。

Objective：To study the effects of the free radical scavenging activity of these formulae for blood-activating and stasis-removing such as Xuefu Zhuyu Decoction（XF）,Shaofu Zhuyu Decoction（SF）,Gexia Zhuyu Decoction（GX）,Shentong Zhuyu Decoction（ST）and Tongqiao Huoxue Decoction（TQ）.Method： Free radical scavenging activity was determined by 2,2-diphenyl-1-picrylhydrazyl（DPPH）method.Result： The result showed that all these formulae had significant effects on scavenging DPPH radical（P0.01）,in a dose-dependent manner..The free radical scavenging capacity on DPPH were in the following descending order：GXTQSFSTXF.The scavenging ability in total formula was better than in ethanol precipitant and supernatant.Conclusion：Validation studies proved that the enzyme-linked immunosorbent assay for DPPH had good reproducibility and feasibility.The method could be used as high throughput screening for Chinese herbs and traditional chinese medicine formulae.