雷公藤醇提物对骨髓细胞增殖和凋亡的影响

Effects of Ethanol Extraction of Tripterygium wilfordiion Proliferation and Apoptosis in Bone Marrow Cells

目的:观察雷公藤醇提物对大鼠骨髓细胞增殖、周期和凋亡的影响。方法:体外培养大鼠股骨骨髓细胞,经不同浓度的雷公藤醇提物作用后,采用MTT比色法检测对骨髓细胞增殖的影响;采用流式细胞仪PI单标法检测对骨髓细胞周期的影响;采用流式细胞仪Annexin V-FITC/PI双标法检测对骨髓细胞凋亡的影响。结果:雷公藤醇提物可明显抑制正常骨髓细胞增殖,并且此抑制作用呈明显的时间和剂量依赖性。细胞周期进程的结果显示,与正常对照组相比,雷公藤组G0/G1期细胞比例显著增多,S期和G2/M期细胞比例显著减少,细胞增殖指数显著降低,早期凋亡率显著升高。结论:雷公藤醇提物可明显抑制骨髓细胞增殖,使骨髓细胞阻滞于G0/G1期,并能诱导细胞凋亡。

Objective：To study effects of ethanol extraction of Tripterygium wilfordii Hook.F.（TWH）on cell proliferation,cell cycle and apoptosis in bone marrow cells（BMCs）in vitro.Method： BMCs were cultured in vitro treated with different concentrations of ethanol extraction of TWH.MTT assay was used to evaluate the cell proliferation.The cell cycle and apoptosis rate were determined by FCM with PI and AnnexinⅤ/PI staining.Result： Viability of BMCs was significantly reduced by ethanol extraction of TWH in a time-and dose-dependent manner.Compared with control group,more BMCs were arrested at G0/G1 phase by TWH.Early Apoptosis rate was increased significantly.Conclusion：Ethanol extraction of TWH could notably inhibit the prolifteration of BMCs,arrest BMCs at G0/G1 phase and induce apoptosis of BMCs.