乳香、没药提取物及其配伍对血小板聚集与抗凝血酶活性的影响

Effect of Extracts from Olibanum and Myrrha and Their Compatibility on Platelet Aggregation and Antithrombin Activity

目的:通过乳香、没药水提物、挥发油及其配伍组合体外对ADP诱导的家兔血小板聚集及对凝血酶时间影响研究,评价其配伍前后的效应变化与量效关系。方法:采用体外二磷酸腺苷(ADP)诱导的血小板聚集实验观察乳香、没药提取物及不同配伍组合的抗血小板聚集活性及量效关系;采用凝血酶时间(TT)法观察乳香、没药提取物及不同配伍组合对凝血酶的影响及量效关系;采用等效线法评价两药配伍的药效相互作用。结果:血小板聚集实验表明:乳香、没药提取物及其不同配伍组合均能显著或非常显著抑制ADP诱导的家兔血小板聚集,且两药配伍后具有协同增效作用,其活性强于各单用提取物的活性。TT实验研究结果表明:乳香、没药不同提取物均能显著延长家兔血浆凝血时间,配伍后除乳香水提物与没药水提物配伍具有拮抗作用外,其余配伍组合均具有协同增效作用。结论:乳香、没药的水提物、挥发油及其配伍组合对家兔血小板聚集及凝血酶时间的影响均能产生显著效应,除乳香水提物和没药水提物配伍组合对凝血酶时间的影响具有拮抗作用外,其余配伍组合均呈现出协同增效作用。研究结果为揭示中药药对配伍的科学性和合理性提供借鉴与参考。

Objective：Effects of platelet aggregation and thrombin time in vitro in rabbits by water extracts and volatile oil from Olibanum and Myrrha and their compatibility were studied to evaluate their effect and dose-effect relationship.Method： Extracts and oils from Olibanum and Myrrha and their combination in different compatibility on platelet aggregation induced by adenosine diphosphate（ADP）in vitro,thrombosis by using thrombin time（TT） method and their dose-effect relationship were observed.Interaction effects of two drugs used in compatibility were measured by an isobole method.Result： The results showed that all the extracts and their combinations in different compatibility had significant or very significant inhibitory effects on the platelet aggregation induced by ADP.Compatibility of the extracts had synergism effect and its activity was better than both single-use of extracts.The experimental data of TT indicated that different extracts from Olibanum and Myrrha could all significantly prolong clotting time of blood plasma in rabbits.Except that combination of water extracts of Olibanum： Myrrh（1∶1） had antagonism effect,the others had synergism effect.Conclusion： Water extracts and volatile oil from Olibanum and Myrrha and their combinations in different compatibility all showed significant effect in vitro on platelet aggregation and thrombin time in rabbits.Furthermore,except that compatibility of water extracts of Olibanum： Myrrh（1∶1） had antagonism effect,the other showed synergistic action.The study provided reference for scientific characters and rationality of herbal compatibility.