舒郁颗粒对卒中后抑郁大鼠模型海马单胺类神经递质及炎症细胞因子的影响

The Effect of Shuyu Granules on Monoamine Neurotransmitters and Inflammatory Factors in Hippocampus in Post-stroke Depression Rats

目的:探讨舒郁颗粒对卒中后抑郁(PSD)大鼠模型行为学、海马单胺类神经递质及肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)的影响。方法:对成年雄性SD大鼠采用双侧颈总动脉永久性结扎后予以行为限制的方法制备PSD大鼠模型,将造模成功的雄性SD大鼠随机分为模型组、假手术组、氟西汀组以及舒郁颗粒高、中、低剂量组共6组,分别给予蒸馏水、氟西汀溶液及舒郁颗粒高、中、低(2.4,1.2,0.6 g.kg-1)剂量ig,22 d后观察大鼠自发性行为改变、海马区单胺类神经递质及TNF-α,IL-1β含量的变化。结果:检测大鼠行为学变化,舒郁颗粒高、中、低剂量组与氟西汀组各项指标无明显差异,与模型组比较差异有统计学意义(P<0.05)。检测大鼠脑组织海马区单胺类神经递质及炎症因子的含量,与模型组比较,舒郁颗粒高、中、低剂量组去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)水平上升有显著性差异(P<0.05),IL-1β及TNF-α含量均显著下降(P<0.05),而与假手术组无明显差异;与氟西汀组各项指标无明显差异。结论:舒郁颗粒可明显改善卒中后抑郁大鼠的行为,提高卒中后抑郁大鼠模型海马单胺类神经递质水平,降低炎症细胞因子含量水平。

Objective：To investgate the effect of Shuyu granules on monoamine neurotransmitters and inflammatory factors in hippocampus of post-stroke depression（PSD）rats.Method： PSD model was established by permanent bilateral common carotid artery ligation together with a behavior limitation.The spontaneous behavior was observed and monoamine neurotransmitters in the hippocampus and tumor necrosis factor-α（TNF-α）and interleukin 1β（IL-1β）were detected in 22 days after surgery.Result： Compared with the model group,there were significant differences in the behavior ability of PSD rats in three dose groups of Shuyu granules and Fluoxetine group（P0.05）.Compared with the model group,norepinephrine（NE）,dopamine（DA）,serotonin（5-HT） were significantly increased（P0.05）in the three dose groups of Shuyu Granules.,IL-1β and TNF-α were significantly decreased（P0.05）.Conclusion：Shuyu granuls could increase monoamine neurotransmitters and reduce cytokines and improve the spontaneous behavior in post-stroke depression rats.