抗柯萨奇B病毒性心肌炎胶囊总黄酮对小鼠急性阿霉素心肌损伤的保护作用

Protection of Total Flavonoids in K-CoxB-JN Against Acute Myocardial Injury Caused by Adriamycin in Mice

目的:观察抗柯萨奇B病毒性心肌炎胶囊(K-CoxB-JN)总黄酮对阿霉素所致小鼠急性心肌损伤的作用,并探讨其可能作用机制。方法:选取正常昆明种小鼠175只,随机分为7组,即K-CoxB-JN黄酮高、中、低剂量组(0.8,0.4,0.2 g.kg-1),模型组,心血康组,正常组,原方水煎组,每组25只,每天ig 1次,1周后ip阿霉素20 mg.kg-1建立小鼠急性心肌损伤模型,24 h后眼球取血测定血清心肌酶水平、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,测定心脾指数。结果:K-CoxB-JN总黄酮能显著降低小鼠心肌酶活性,黄酮中剂量组乳酸脱氢酶(LDH)水平有所下降,α-羟丁酸脱氢酶(HBDH)水平降低明显(P<0.01);黄酮高、中剂量组心脏指数明显降低(P<0.01),黄酮中剂量组脾指数有显著性差异(P<0.05)。黄酮高、中剂量组SOD活力明显升高(P<0.01),黄酮中剂量组MDA含量有显著性降低(p<0.01),以黄酮中剂量效果最为明显。结论:K-CoxB-JN总黄酮对小鼠急性心肌损伤有保护作用,其作用机制可能与抗氧化、清除氧自由基有关。

Objective：to observe protection of total flavonoids in anti-coxsackie B complex capsule（K-CoxB-JN） against acute myocardial injury caused by adriamycin in mice and to discuss the feasible mechanism.Method： One hundred and seventy five Kunming mice were selected and diveded into 7 groups,including high dose group of flavonoid（0.8 g·kg-1）,middle dose group of flavonoid（0.4 g·kg-1）,low dose group of flavonoid,model group（0.2 g·kg-1）,Xinxuekang group,blank group and original formula group.,there 25 mice in each group are taken.After a week,model of acute myocardial injury was established by adriamycin injection of 20 mg·kg-1.Blood was collected after 24 h of model building,then determination of myocardial enzyme like SOD and MDA were performed as well as indexes of heart and spleen.Result： Activity of myocardial enzymes was decreased drastically with total flavonoids in K-CoxB-JN,as well as indexes of heart and spleen,especially middle dose group of flavonoid.Conlusion：Total flavonoids in K-CoxB-JN do prectect mice against acute myocardial injury.The possible meachanism may be related to antioxidation and scavenging of oxygen free radicals.