天王补心丸镇静安神作用的研究

Experimental Study on Sedative and Hypnotic Effects of Tianwang Buxin Wan

目的:观察天王补心丸镇静催眠的药理作用。方法:选用ICR小鼠,随机分为5组:天王补心丸高、中、低剂量组,空白对照组,安神健脑液组。天王补心丸剂量组分别在(生药)3.24,1.62,0.81 g.kg-1剂量下连续ig给药7 d,测定药物对正常和阴虚模型小鼠自主活动的影响,以及对阈下、阈剂量戊巴比妥钠对催眠作用的影响。结果:测定5 min内小鼠自主活动数/次、睡眠率/%、睡眠潜伏期、维持睡眠时间。小鼠ig天王补心丸,对正常小鼠的自主活动没有明显影响,但能减少阴虚模型小鼠自主活动次数。对戊巴比妥钠所致睡眠有一定的协同作用,缩短小鼠睡眠潜伏期,延长小鼠睡眠时间。结论:天王补心丸具有一定的镇静安神作用。

Objective：To investigate the sedative and hypnotic effects of Tianwang Buxin Wan（TWBXW）on mice model.Method： ICR rats were divided into five groups randomly,three dose groups of TWBXW（3.24,1.62,0.81 g·kg-1）,control group and Annao Jianshen group.Spontancous locomotor activities of mice in five minutes,precentage of sleep,sleep latency,maintaining sleep were evaluated.The effects of TWBXW on spontaneous locomotor activities in normal mice group and yin deficiency mice group were observed.Hypnotic effects were assayed by cooperated with pentobarbital sodium.Result： There was no direct influence of TWBXW on the spontaneous locomotor activity in normal mice.But it could significantly decrease the spontaneous locomotor activity of mice with yin deficiency syndrome.TWBXW could reduce sleep latency and extend sleep time induced by pentobarbital sodium.Conclusion：TWBXW showed certain sedative and hypnotic effects.