微波光波组合辐射辅助提取赤小豆黄酮的机制分析

Study on extraction mechanism of total flavone from red phaseolus bean by microwave and light wave radiation

考察赤小豆总黄酮的微波光波组合辐射提取法,并对提取机理进行分析。发现微波光波提取法以功率800W(微波55%与光波45%)加热6min后,70%乙醇萃取得总黄酮,总黄酮的提取率为1.24%,与相应的常规加热回流提取法接近,该法有操作简单快速、成本低等优点。利用荧光显微镜FM、IR对微波提取机理进行初步研究表明,微波光波可能是从对植物组织细胞结构的影响上来改善次生代谢产物的提取效率。

The extraction method and mechanism of total flavonoids from red phaseolus bean had been studied using microwave and light wave radiation. The total flavonoids were extracted with ethyl alcohol after heating the sample for 6min with power 800W （microwave 55% and light wave 45%）. The extraction rate of total flavonoids was 1.24%,which closes with the conventional extraction process. This method has many merits,such as simple, rapid,low cost,and so on. Extraction mechanism was investigated preliminarily with high magnification fluorescence microscope FM,IR. It indicated that the improvement of extraction efficiency of secondary metabolite maybe due to the plant tissue cell structure that were broken by microwave and light wave.