匹立尼酸对肝脏缺血-再灌注大鼠血清TNF-α和IL-1β的影响

Effects of pirinixic acid on serum TNF-α and IL-1β in rats with hepatic ischemia-reperfusion injury

目的观察匹立尼酸（Wy14643）对肝脏缺血-再灌注大鼠血清肿瘤坏死因子α（TNF-α）和白细胞介素1β（IL-1β）的影响。方法SD雄性大鼠30只，体重220-280g，随机均分为五组：W1、W2、W2组、缺血-再灌注组（LR组）和假手术组（S组）。阻断左、中侧肝动脉及其门静脉（缺血约70％）90min后再灌注建立大鼠缺血-再灌注模型，S组操作同前但不阻断血管。缺血前1h，W1、W2、W3组分别经腹腔注射Wy146431、5、10mg／kg，I-R组和S组注射等容积生理盐水。4h后取腹主动脉血测定血清TNF-α和IL-1β水平。结果再灌注4h后，S组血清TNPα和IL-1β水平明显低于其它四组（P〈0．05）；W2、W3组明显低于LR组（P〈0．05）。结论预先给予匹立尼酸可降低肝脏缺血一再灌注后血清TNF-α和IL-1β水平。

Objective To observe the effects of pirinixic acid （Wy14643） on serum TNF-α and IL-1β in rats with hepatic ischemia-reperfusion （I-R） injury. Methods Thirty male Sprague-Dawley rats weighing 220-280 g were equally randomized into five groups： Wy14643 1 mg/kg ＋ I R group （group W1 ）, Wy14643 5 mg/kg ＋ I-R group （group W2）, Wy14643 10 mg/kg ＋I-R group （group W3）, sham operation group （group S） and I-R group （group I-R）. Hepatic I-R injury model was established by clamping blood supply of the left and median lobes of the liver with vascular clamps for 90 min. Rats in group S were done the same operation without clamping blood vessels. Rats in groups W1, W2 and W3 were intraperitoneally administered with the dose of Wy14643 1, 5 and 10 mg/kg 60 min before ischemia, respectively. In groups S and I-R, rats were given the same volume of saline at the same time. Blood samples were taken from abdominal aorta 4 h after reperfusion to measure the serum levels of TNF-α and IL-1β. Results Four hours after hepatic I-R, group S had lower serum TNF-α and IL-1β compared with groups W1 , W2, W3 and I-R （P〈0. 05）. Groups W2 and W3 had lower serum TNF-αand IL-1βcompared with group I-R （P〈0. 05）. Conclusion Pretreatment with pirinixic acid can reduce the serum levels of TNF-α and IL-1β in rats with hepatic ischemia-reperfusion injury.