桑叶黄酮对胰岛素抵抗大鼠氧化应激影响

Effect of mulberry flavonoids on oxidatie stress among diabetic rats with insulin resistance

目的观察桑叶黄酮对2型糖尿病胰岛素抵抗大鼠氧化应激作用,并探讨其改善胰岛素抵抗的分子机制。方法高脂饮食饲养成年雄性SD大鼠8周诱导大鼠胰岛素抵抗模型,随机分为糖尿病模型组(MOD组)和桑叶黄酮治疗组(MLF组),对照组为正常SD大鼠(NOR组);测量各组大鼠空腹血糖(FPG)、胰岛素(FINS)及血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,计算胰岛素敏感指数(ISI)和胰岛素抵抗指数(IR I)。结果与NOR组比较,MOD组MDA升高,为(8.73±0.85)nmol/L,SOD、GSH-Px活性均降低,分别为(226.45±17.54)、(529.03±69.56)U/mL;与MOD组比较,MLF组能降低病鼠FINS和IR I,分别为(25.83±2.1)mU/L和(8.86±0.96),提高ISI,为(5.08±0.09),降低FPG和MDA含量,分别为(7.95±1.85)mmol/L和(6.82±0.79)nmol/mL,增强SOD活性,为(297.85±22.16)U/mL。结论桑叶黄酮能改善2型糖尿病胰岛素抵抗大鼠高胰岛素血症,降低血糖,提高胰岛素敏感性,降低氧化应激水平,具有抗氧化能力。

Objective To observe the effect of mulberry flavone on oxidative stress in type 2 diabetes rats with insulin resistance and to explore molecular mechanism of mulberry flavone against insulin resistance.Methods The adult male SD rats were fed with high-fat diet for 8 weeks,and then randomly divided into model group with type 2 diabetes（MOD group）,mulberry leaves flavone treatment group（MLF）,and normal control group（NOR group）.We observed levels of fasting plasma glucose（FPG）,fasting insulin（FINS）,serum malondialdehyde（MDA）,superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） activity,insulin sensitivity index（ISI）,and insulin resistance index（IRI）.Results Compared with the normal group,serum MDA（8.73±0.85 nmol/L） was significantly increased;SOD（226.45±17.54 U/ml） and GSH-Px（529.03±69.56 U/ml） activity were significantly lower in model group.Compared with model group,mulberry flavone could significantly reduce FINS（25.83±2.1 mU/L） and IRI（8.86±0.96）,increase ISI（5.08±0.09）,lower FPG（7.95±1.85 mmol/L）,and increase SOD（297.85±22.16 U/ml） activity,decrease MDA（6.82±0.79 nmol/ml） content.Conclusion Mulberry flavone can improve insulin resistance in type 2 diabetic rats with hyperinsulinemia,lower plasma glucose,improve insulin sensitivity,and resist oxidative stress.