甲醛和甲苯联合染毒致小鼠脑组织氧化损伤作用

Oxidative damages in brain induced by joint exposure to formaldehyde and toluene in mice

目的研究甲醛和甲苯联合染毒致小鼠脑组织氧化损伤作用,探讨其联合毒性效应的类型。方法采用静式吸入染毒,将小鼠暴露于不同浓度的甲醛、甲苯及两者的混合气体中。染毒结束后测定脑组织中活性氧(ROS)、谷胱甘肽(GSH)、丙二醛(MDA)、超氧化物歧化酶(SOD)和一氧化氮合酶(NOS)。结果甲醛和甲苯染毒可致小鼠脑组织ROS、MDA含量和NOS活力增高,SOD活力和GSH含量降低(P<0.01),其中联合组(5 mg/m3+2 000 mg/m3)的影响最为明显;二者联合染毒致小鼠脑组织氧化损伤存在交互作用(P<0.05),且表现为协同。结论甲醛和甲苯吸入可致小鼠脑组织氧化损伤,二者联合染毒具有一定的协同作用。

Objective To study combined toxic effect of formaldehyde and toluene exposure on oxidative damage of brain in mice.Methods The treatments were administered by static inhaling formaldehyde or/and toluene.The activity of superoxide dismutase（SOD）,nitric oxide synthase（NOS）,the contents of reactive oxygen species（ROS）,glutathione（GSH）,and malondialdehyde（MDA） in the brain of the mice were determined.Results When the mice were jointly exposed to formaldehyde and toluene,the contents of ROS and MDA,the activity of NOS in brain were increased and the contents of GSH and the activity of SOD were decreased（P0.01）.There were interactions between formaldehyde and toluene in ROS,NOS,SOD,GSH,and MDA（P0.05）.Conclusion Formaldehyde and toluene could induce oxidative damage in brain of mice.The joint toxicity of formaldehyde and toluene is synergistic.