枸杞多糖对辐照大鼠生殖系统损伤拮抗作用

Antagonistic effect of LBP on damage of reproductive system induced by sub-chronic partial 60Co γ-ray radiation in male rats

目的探讨枸杞多糖(LBP)对60Co γ射线局部辐射导致雄性大鼠生殖系统损伤的影响。方法 84只Wistar雄性大鼠,随机分为对照组,辐射后1、7、14 d组,LBP+辐射后1、7、14 d组,灌胃后每周一至周五进行60Coγ射线局部辐射,共4周,分别于末次辐射后1、7、14 d处死大鼠。观察辐射后大鼠性能力,计算精子计数和存活率,检测睾丸组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果辐射后7 d大鼠扑捉潜伏期和射精潜伏期分别为(449.38±59.01)和(463.25±53.77)s,明显高于对照组(272.75±57.63)和(278.50±54.33)s(P<0.01),扑捉次数、射精次数及精子数量也明显减少(P<0.01);辐射后14 d大鼠睾丸组织中SOD活力和MDA含量分别为(124.46±5.32)U/mgprot和(2.24±0.16)nmol/mgprot,明显高于对照组(P<0.01);LBP干预各组指标较辐射组明显改善,辐射后7 d扑捉潜伏期和射精潜伏期分别降低至(312.88±31.33)和(325.00±31.05)s,SOD活力升至(148.44±8.71)U/mgprot,而MDA降至(1.67±0.10)nmol/mgprot,接近于对照组水平。结论枸杞多糖对60Co γ射线局部辐射导致雄性大鼠生殖系统损伤有明显拮抗作用,能促进损伤恢复。

Objective To study the effect of Lycium barbarum polysaccharides（LBP） on reproductive system damage induced by sub-chronic partial 60Co γ-radiation in male rats.Methods Totally 84 Wistar male rats were randomly divided into 1 control group,3 radiation groups,and 3 LBP＋ radiation groups.After LBP gavage for 7 days,the treatment groups were exposed to 60Co γ-ray local irradiation 5 days in a week for 4 weeks with the target distance of 80cm and the total dose of 2.3 Gy for each rat.The irradiation location was lower abdomen and the places above the anterior superior iliac crest was covered with 3 mm thick lead grid.During the irradiation,the rats in control group were administrated with equal quantity of normal saline.The rats in radiation groups and LBP＋radiation groups were killed by severing the femoral artery at 1st,7th,and 14th day of the radiation and their sexual ability was observed and the sperm count and viability,the contents of superoxide dismutase（SOD） and malondialdehyde（MDA） were determined.Results The average mount of latency and ejaculation latency in radiation groups were 449.38 s and 463.25 s and significantly higher than those of the control group（272.75 s and 278.50 s）（P0.01）.Mount frequency,ejaculation frequency,and sperm count of the radiation group were also significantly different from those of the control group.SOD and MDA in model groups were 124.46 U/mg prot and 2.24 nmol/mg prot but those of the contorl group were 155.11 U/mg prot and 1.30 nmol/mg prot with significant differences（P0.01）.The mount latency and ejaculation latency in LBP＋radiation groups reduced to 312.88 s and 325.00 s and the SOD activity and MDA content were 148.44 U/mg prot and 1.67 nmol/mg prot and similar to those of the control group.Conclusion LBP has obvious antagonistic effect on reproductive system damage induced by 60Co γ-sub-chronic partial radiation and can promote the recovery of the damage in male rats.