虾青素对^(60)Coγ射线所致小鼠氧化损伤的保护作用

Protective effects of astaxanthin against oxidative damage induced by ^(60)Co γ-ray irradiation

目的探讨虾青素对60Coγ射线诱导的小鼠氧化损伤的保护作用。方法 50只昆明种小鼠随机分为5组,即对照组、模型组和虾青素高、中、低剂量组(10.2、2.0和4.0mg/kg)。辐照前,各剂量组每日经口灌胃不同浓度的虾青素,模型组和对照组则给予等体积植物油,连续30天。30天后,除对照组外,其余各组给予8 Gy 60Coγ射线全身一次性照射。照射后第4天处死小鼠,取肝细胞进行彗星实验以观察细胞DNA损伤,制取肝匀浆测定MDA含量、SOD和GSH-Px活性,同时进行外周血粒细胞和骨髓有核细胞计数。结果与对照组相比,模型组MDA含量显著升高(P<0.01),SOD和GSH-Px活性显著降低(P<0.01);与模型组相比,虾青素各组MDA含量显著降低(P<0.01),SOD活性显著升高(P<0.01),高剂量组GSH-Px活性显著升高(P<0.01)。模型组DNA损伤指标均显著高于对照组(P<0.01),而虾青素各组DNA损伤显著低于模型组(P<0.01),且随着虾青素剂量的增加,细胞拖尾率和OTM逐渐减少。与对照组相比,模型组粒细胞和骨髓有核细胞计数显著降低(P<0.01),而虾青素各组粒细胞和中、高剂量组骨髓有核细胞显著高于模型组(P<0.01)。结论虾青素对于辐射诱导的氧化损伤具有保护作用。

Objective To investigate the protection effect of haematococcus pluvialis（containing astaxanthin） against the impairment of anti-oxidative system and DNA damage in mice induced by 60Co γ-rays.Methods Fifty mice were randomly divided into five groups,i.e.three haematococcus pluvialis groups（41.7,83.3 and 166.7mg/kg in vegetable oil,respectively）,control group and model group（vegetable oil only）.All mice except control group were irradiated by 8 Gy 60Co γ-rays 30 days later,and executed in the 4th day after irradiation.Liver cells were collected for the analysis of the integrity of DNA by comet assay,as well as MDA contents,SOD and GSH-Px activities in liver by commercial kits.Peripheral granulocyte and bone marrow nucleated cells were counted by hematocyte counter.Results MDA contents of model group were higher than those of control group（P0.01）,and SOD,GSH-Px activities of model group were lower than those of control group（P0.01）.Compared with the model group,MDA contents were decreased（P0.01）,and SOD and GSH-Px activities were increased（P0.01） in all haematococcus pluvialis groups,especially in the high haematococcus pluvialis group,and the more haematococcus pluvialis in the diet of mice,the lower rate of comet tail and OTM value were shown（P0.01）.Furthermore,the counts of peripheral granulocyte and bone marrow nucleated cells of model group were lower than those of the control group,while the counts of peripheral granulocyte and bone marrow nucleated cells of medium and high haematococcus pluvialis groups were increased significantly when compared with the model group（P0.01）.Conclusion Astaxanthin might have some protective effect against oxidative impairment and DNA damage induced by 60Co γ-rays in mice.