番茄红素对高脂血症大鼠血细胞和纤溶活性的影响

Effects of lycopene on blood cells and fibrinolytic activity in hyperlipidemic rats

目的探讨番茄红素对高脂血症大鼠血细胞及纤溶活性的影响。方法成年雄性SD大鼠40只,根据总胆固醇(TC)水平随机分为5组,每组8只:正常对照组、高脂模型组、氟伐他汀钠10mg/kg bw组、番茄红素11mg/kg bw和44mg/kg bw组。正常对照组饲基础饲料,其余组饲高脂饲料,实验第2、3周氟伐他汀钠和番茄红素灌胃处理。检测TC、甘油三酯(TG)、低密度脂蛋白-胆固醇(LDL-C)、高密度脂蛋白-胆固醇(HDL-C)、超氧化物歧化酶活力(SOD)和丙二醛水平(MDA)、血细胞参数、血小板α颗粒膜蛋白(GMP-140)、组织型纤溶酶原激活物(tPA)、组织型纤溶酶原激活物抑制剂-1(PAI-1),计算tPA/PAI-1比值和动脉粥样硬化指数(AI);HE染色观察主动脉弓的病理变化。结果高脂饲料喂养1周后大鼠形成高脂血症。与模型组比较,番茄红素组的TC、TG、LDL-C和MDA下降,SOD升高;白细胞计数、红细胞体积、血小板体积、GMP-140和PAI-1下降,红细胞计数和tPA上升;AI显著下降,主动脉弓内膜变薄,泡沫细胞减少。番茄红素44mg/kg bw剂量组作用明显。结论番茄红素可能通过降低血脂和抗氧化而保护高脂血症大鼠的血细胞和促进纤溶活性,减轻主动脉病变程度。

Objective To explore the effects of lycopene on blood cells and fibrinolytic activity in hyperlipidemic rats.Methods Forty adult male SD rats were divided randomly into five groups：normal control group,high fat model group,fluvastatin sodium group,lycopene low-dose group and lycopene high-dose group based on the level of serum TC.Normal control group was fed with normal animal diet,other groups were fed with high fat diet.Fluvastatin sodium and lycopene were given by intragastric administration in the last two weeks of experiment.The level of serum lipid,SOD,MDA,blood cell parameters,platelet（granule membrane protein（GMP-140）,tissue plasminogen activator（t-PA） and plasminogen activator inhibitor-1（PAI-1） were measured.The pathological changes of aorta were observed and atherosclerosis index was calculated.Results Hyperlipidemia was induced in rats after feeding high fat diet for one week.In comparison with high fat diet model group,serum TC,TG,LDL-C and MDA were decreased but HDL-C and SOD were increased in lycopene groups.White blood cell count,mean volume of red blood cells and platelets,GMP-140 and PAI-1 were decreased,but red blood cell count and tPA were increased;atherosclerosis index were decreased,foam cells were decreased in intima,pathological changes in aorta were reduced in lycopene groups.The effects were obvious in lycopene 44 mg/kg bw group.Conclusion The effect of lycopene on protecting blood cell,promoting fibrinolytic activity and reducing aortic lesions in hyperlipidemic rats might be the result of reducing blood lipids and improving antioxidation.