巴旦木蛋白提取工艺

Optimization of Protein Extraction from Amygdalus communis L. Seed Kernel

以巴旦木为原料,采用碱提酸沉法和超声波辅助碱液浸提法制备巴旦木蛋白,并对其理化性质进行研究。结果表明:碱提酸沉法提取巴旦木蛋白的最佳工艺条件是料液比1:25、pH10、60℃浸提40min;超声辅助碱液浸提法提取巴旦木蛋白的最佳工艺条件是pH9.0、料液比1:20、提取时间15min、超声功率125kW,在此条件下提取率为37.16%。与碱提酸沉法比较,超声波辅助碱液提取法时间短、得率高。

Orthogonal array design was used to optimize the extraction of protein from almond（Amygdalus communis L.） seed kernel by alkaline extraction and acid precipitation method or ultrasonic-assisted alkaline extraction method.The optimal conditions for protein extraction by alkaline extraction and acid precipitation were obtained as follows： material/liquid ratio 1：25,pH 10.0,extraction time 40 min,and extraction temperature 60 ℃.The optimal ultrasonic-assisted alkaline extraction conditions were material/liquid ratio 1：20,pH 9.0,extraction time 15 min,and ultrasonic power 125 kW,resulting in an extraction efficiency of 37.16%.Therefore,the ultrasonic-assisted alkaline extraction method revealed the advantages of shorter extraction time and higher extraction efficiency over the alkaline extraction and acid precipitation method.