生物胺高效液相色谱法测定条件的选择与优化

Optimization of Operating Conditions for HPLC Determination of Biogenic Amines

利用反相高效液相色谱、荧光/二极管阵列检测器对8种生物胺进行测定,通过对流动相选择、流动相梯度洗脱设定、检测器比较、检测波长比较及其他条件的探讨,得出高效液相色谱法测定生物胺的最佳色谱条件。结果确定,采用乙腈-乙酸铵-超纯水作为流动相经梯度洗脱,8种生物胺在30min内全部被分离出且峰形对称无拖尾和前伸现象。改进后的色谱条件可节约样品分析时间,荧光检测器的高选择性和灵敏性能够满足高精确度生物胺检测分析的要求。

In order to establish a reversed-phase HPLC method for the determination of 8 biogenic amines,mobile phase composition,elution mode,detector type and detection wavelength were optimized.Gradient elution using a mobile phase composed of acetonitrile,ammonium acetate and ultra-pure water proved optimal,and 8 biogenic amines were completely separated within 30 min,showing symmetrical peak shapes without tails and extension.The optimization resulted in a time-saving analytical method.Moreover,fluorescence detector（FLD） presented higher selectivity and sensitivity when compared with diode array detector（DAD） so that it can meet the requirements for accurate determination of biogenic amines.