摘要
cDNA文库因不含内含子而便于克隆和大量扩增，利用多种筛选方法并结合生物信息学软件获得阳性克隆，对于从分子水平上深入研究广州管圆线虫病的致病机制及诊断方法具有重要意义。该文综述了广州管圆线虫各期cDNA文库特异基因筛选的研究进展。
This paper reviewed the research progress on screening of the specific genes from Angiostrongylus cantonensis cDNA library. The cDNA library is easy to clone and amplify for lack of introns. Obtaining positive clones using a variety of screening methods combined with softwares of bioinformatics is of significance to deep on the researches on pathogenic mechanism and diagnostic methods of Angiostrongylus cantorvensis on molecular level.
出处
《国际医学寄生虫病杂志》
CAS
2011年第5期311-314,共4页
International JOurnal of Medical Parasitic Diseases
基金
基金项目：国家973基金（2010cB530004）和国家自然科学基金重点项目（2005U0632003）基金联合资助
关键词
广州管圆线虫
CDNA文库
筛选
Angiostrongylus cantonensis
cDNA library
Screening
