摘要
目的:观察红花有效成分羟基红花黄色素A(HSYA)缓解血小板激活因子(PAF)诱发的内皮细胞炎症介质表达升高的作用。方法:培养Eahy926脐静脉内皮细胞株,加入HSYA,以PAF刺激后提取总RNA,RT-PCR法检测白细胞介素1-β(IL-1β)、IL-6、细胞间黏附分子-1(ICAM-1)及血管细胞黏附分子-1(VCAM-1)mRNA表达水平的变化。结果:PAF刺激后内皮细胞IL-1β、IL-6、ICAM-1及VCAM-1 mRNA表达水平升高,HSYA可缓解这种变化。结论:HSYA能够缓解PAF诱发的血管内皮细胞炎症介质IL-1β、IL-6、ICAM-1及VCAM-1 mRNA表达的升高。
Objective:To observe the inhibitory effect of Hydroxysafflor yellow A(HSYA) on endothelium inflammatory factors expression elevation triggered by platelet activating factor(PAF).Methods:Umbilicus vein endothelium cell line Eahy926 was cultured.HSYA and then PAF was added to this cell.After RNA isolation IL-1β,IL-6,ICAM-1 and VCAM-1 mRNA expression levels were assayed by RT-PCR technology with actin as internal control.Results:Endothelium IL-1β、IL-6、ICAM-1、VCAM-1 mRNA expression levels increased after PAF addition and HSYA can attenuate these changes.Conclusion:HSYA is effective to inhibit endothelium IL-1β,IL-6,ICAM-1 and VCAM-1 mRNA expression elevation induced by PAF.
出处
《心肺血管病杂志》
CAS
2011年第5期429-432,共4页
Journal of Cardiovascular and Pulmonary Diseases
基金
国家自然科学基金资助项目(30572344)
北京市自然科学基金资助项目(7072025)