FQ-PCR技术在儿童EB病毒感染早期诊断中的应用

Application of FQ-PCR technology in early diagnosis of children with EB virus infection

目的:探讨荧光定量PCR(FQ-PCR)技术在儿童Epstein-Barr Virus(EBV)感染早期诊断中的应用。方法:将2009年4月-2011年2月份收治的186例表现为发热、咽峡炎、淋巴结肿大等患儿采用实时荧光定量PCR法检测外周血淋巴细胞中EBV-DNA。结果:186例患儿中,外周血EBV-DNA阳性66例,其中男39例,女27例;受EBV感染的患儿,近1/4阳性病例在3 d内测出,超过4/5的阳性病例在发病7 d内即可明确诊断。结论:FQ-PCR技术对儿童EBV感染的检测具有早期、快速、准确等优点,值得推广应用。

Objective： To explore value of FQ-PCR technique in early diagnosis of the children infected by EB virus.Methods： 186 children with fever,pharyngitis or lymphadenopathy et al,received from April in 2009 to February in 2011,were selected to conduct detection of EBV-DNA in peripheral blood cells by FQ-PCR method. Results： There are 66 positive cases,including 39 boys and 27 girls.A quarter of them can be diagnosed in 3 days,overrun 80% of them can be diagnosed in 7 days.Conclusion： Children infected by EB virus have complex clinical symptoms,which is hard to diagnose early;FQ-PCR technique is the best method to check EBV-DNA.It has many virtues and worth application.