TB-IGRA试验在调查本地健康中老年人群结核分支杆菌特异性γ-干扰素本底值的应用

Usefulness of mycobacterium tuberculosis-specific interferon-γ release assay in investigating the background value of it in healthy elderly population in the region

目的:探索本地区45岁以上健康中老年人群血浆结核分支杆菌特异性γ-干扰素本底值,为TB-IGRA定量试验广泛应用于结核病的临床诊断、疗效观察提供本底值参照依据。方法:选取100位45岁以上健康中老年人,结核分枝杆菌(MTB)γ-干扰素体外释放定量试验测定其血浆中特异性γ-干扰素含量。结果:盐城市100位健康中老年人血浆特异性γ-干扰素含量为0.1 pg/ml～7.1 pg/ml,中位数为1.1 pg/ml。结论:本地区健康中老年血浆中特异性γ干扰素含量存在一定的本底值,在使用TB-IGRA定量试验方法进行结核病临床诊断、疗效观察时要充分考虑健康人群本底值带来的影响。

Objective： To explore the background value of Mycobacterium Tuberculosis-specific Interferon-γ in healthy population with age over 45 years in Yancheng city and to provide reference for TB-IGRA test widely used in clinical diagnosis of TB and efficacy observation.Methods： Mycobacterium tuberculosis（MTB） interferon-γ test in vitro quantitative form was used to detect the specific interferon-α level in plasma of 100 selected healthy elderly over 45 years.Results： Plasma concentration of interferon-γ in 100 selected elderly was 0.1 pg/ml^7.1 pg/ml,with median of 1.1 pg/ml.Conclusion： There is a certain background value of MTB-specific interferon-γ in plasma of the elder over 45 years in the region,and the impact of which should be given full consideration when using the TB-IGRA quantitative test methods for clinical diagnosis of tuberculosis and efficacy observation.