苏州地区2010年手足口病流行情况分析和实验室检测

The epidemiology trend and the laboratory test of hand,foot and mouth disease in Suzhou city in 2010

目的:通过对2010年苏州地区手足口病病例进行病原体检测并进行流行特征分析,为手足口病的防控提供科学依据,同时评估实时荧光定量PCR在手足口病防控中的应用价值。方法:临床诊断为手足口病病例的咽拭子标本1711份,首先采用实时荧光定量PCR筛选出总肠道病毒阳性的标本,再分别使用CA16和EV71特异性引物进行荧光定量PCR检测。结果:手足口病全年均可发病,以4月-7月为发病高峰季节。发病率最高的年龄段为1岁～4岁,男性多于女性。重症病例主要由EV71引起。结论:苏州市的手足口病毒以EV71为主。实时荧光定量PCR法可用于手足口病的病原体的快速检测和分型,有利于感染者的早发现、早隔离,对手足口病的监测及防控具有重要作用。

Objective： To analyze pathogen surveillance data of hand,foot and mouth disease（HFMD） in Suzhou in 2010,to provide scicentific references for prevention and contrl policy and to study the application value of fluorescence real-time polymerase chain reaction（PCR） in detecting nucleic acid of the virus causing HFMD.Methods： 1711 throat swabs were collected from patients who were clinical diagnosed as HFMD,nucleic acid were purified and identified by fluorescence real-tim PCR,then EV71 and CoxA16 RNA was detected in the total enterovirus positive samples.Results： HFMD could happen in all four seasons,but most cases occurred from April to July.Children aged 1～4 years old were high risk group,and infected boys are much more than that of girls.EV71 accounted for most of the cases of serious conditions.Conclusion： EV71 was dominant virus in HFMD in Suzhou city in 2010.Fluorescence real-time PCR can be used as a rapid diagnostic method because of its sensitivity,specificity and efficiency,which are very important in prevention and control of enterovirus infection.